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DNA barcoding reveals mislabeling of imported fish products in Nansha new port of Guangzhou, Guangdong province, China

文献类型： 外文期刊

作者： Yan, Shuai 1 ; Lai, Guiyan 2 ; Li, Li 2 ; Xiao, Hao 2 ; Zhao, Ming 2 ; Wang, Ming 2 ;

作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Minist Agr, Key Lab South China Sea Fishery Resources Exploit, Guangzhou 510300, Guangdong, Peoples R China

2.Nansha Entry Exit Inspect & Quarantine Bur, Guangzhou 511457, Guangdong, Peoples R China

3.Guangdong Inst Standardizat, Guangzhou 510220, Guangdong, Peoples R China

关键词： COI;DNA barcoding;Mislabeling;Species identification;Alepes apercna

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

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收录情况： SCI

摘要： In the present study, we employed a DNA barcoding approach to authenticate the species of fish imported via one port in China. The fish were identified as smallmouth scad based on morphological characteristics, Alepes apercna (Perciformes, Carangidae), but were labeled as Rastrelliger brachysoma (Perciformes, Scombridae). Fragments of the partial mitochondrial cytochrome c oxidase 1 (COI) gene were sequenced from 12 specimens, and their phylogenetic relationship was subsequently examined. The phylogenetic analysis demonstrated that all of the individuals formed a monophyletic cluster with high bootstrap values and were placed in a sister group with the ancestor of Alepes vari and Alepes melanoptera. The K2P genetic distances at an intraspecific level were significantly smaller than those at an interspecific level. Our results indicated that the fish were A. apercna, rather than R. brachysoma, which confirms the morphological analysis. This study presents a practical demonstration of the use of DNA barcoding to prevent fraud in international trade. (C) 2016 Elsevier Ltd. All rights reserved.

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