文献类型： 外文期刊

作者： Tang, H. ¹ ; Wei, B. ¹ ; Huang, S. ¹ ; Ahmed, M. S. ¹ ; Yang, H. ² ; Lou, K. ³ ; Shi, Y. ³ ; Feng, X. ¹ ; Li, C. ¹ ;

作者机构： 1.Beijing Inst Technol, Sch Life Sci, Beijing 100081, Peoples R China

2.Shihezi Univ, Key Lab Green Proc Chem Engn Xinjiang Bingtuan, Sch Chem & Chem Engn, Shihezi 832003, Peoples R China

3.Xinjiang Acad Agr Sci, Inst Microbiol, Urumqi 830000, Peoples R China

关键词： beta-glucuronidase;glycosylation;ionic liquids;thermostability

期刊名称：CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY （ 影响因子：1.582； 五年影响因子：2.203 ）

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收录情况： SCI

摘要： The purification of glycosylated recombinant beta-glucuronidase from Pichia pastoris GS115 (PGUS-P) was investigated by a novel two-step process: ammonium sulfate precipitation and molecular sieve chromatography. The highest purification fold obtained was 66.79. The catalytic properties of glycosylated PGUS-P in hydrophobic ionic liquids (ILs)/buffer biphasic system were investigated. A 2.2-fold enhancement in the catalytic efficiency was observed using 50% (v/v) 1-butyl-3-methylimidazolium hexafluorophosphate, in comparison with the acetate buffer medium. When compared with the glycosylated PGUS-P, the deglycosylated enzyme at T-55 and T-65 exhibited low activity and low thermal stability in both ILs and acetate buffer. It was also observed that ILs had effect on the pH profile of deglycosylated PGUS-P - the optimum pH was extended from 5.0 (acetate buffer) to 5.0-7.0 (ILs). Therefore, this study indicates that the glycosylation of PGUS-P plays an important role in both catalytic activity and stability in hydrophobic ionic liquids (ILs)/buffer biphasic system.