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Immunogenicity of adenovirus-derived porcine parvovirus-like particles displaying B and T cell epitopes of foot-and-mouth disease

文献类型： 外文期刊

作者： Pan, Qunxing 1 ; Wang, Hui 2 ; Ouyang, Wei 1 ; Wang, Xiaoli 1 ; Bi, Zhenwei 1 ; Xia, Xingxia 1 ; Wang, Yongshan 1 ; He, K 1 ;

作者机构： 1.Natl Ctr Engn Res Vet Bioprod, Inst Vet Med, Jiangsu Acad Agr Sci, Key Lab Vet Biol Engn & Technol,Minist Agr, Nanjing 210014, Peoples R China

2.Zaozhuang Bur An

关键词： Hybrid virus-like particles;Porcine parvovirus;Foot and mouth disease virus;Molecular mimicry

期刊名称：VACCINE （ 影响因子：3.641； 五年影响因子：3.816 ）

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收录情况： SCI

摘要： Virus-like particles (VLPs) vaccines combine many of the advantages of whole-virus vaccines and recombinant subunit vaccines, integrating key features that underlay their immunogenicity, safety and protective potential. We have hypothesized here the effective insertion of the VP1 epitopes (three amino acid residues 21-40,141-160 and 200-213 in VP1, designated VPe) of foot-and-mouth disease (FMDV) within the external loops of PPV VP2 could be carried out without altering assembly based on structural and antigenic data. To investigate the possibility, development of two recombinant adenovirus rAd-PPV:VP2-FMDV:VPe a or rAd-PPV:VP2-FMDV:VPe b were expressed in HEK-293 cells. Out of the two insertion strategies tested, one of them tolerated an insert of 57 amino acids in one of the four external loops without disrupting the VLPs assembly. Mice were inoculated with the two recombinant adenoviruses, and an immunogenicity study showed that the highest levels of FMDV-specific humoral responses and T cell proliferation could be induced by rAd-PPV:VP2-FMDV:VPe b expressing hybrid PPV:VLP5 (FMDV) in the absence of an adjuvant. Then, the protective efficacy of inoculating swine with rAd-PPV:VP2-FMDV:VPe b was tested. All pigs inoculated with rAd-PPV:VP2-FMDV:VPe b were protected from viral challenge, meanwhile the neutralizing antibody titers were significantly higher than those in the group inoculated with swine FMD type O synthetic peptide vaccine. Our results clearly demonstrate the potential usefulness of adenovirus-derived PPV VLPs as a vaccine strategy in prevention of FMDV. (C) 2015 Elsevier Ltd. All rights reserved.

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