Effects of norfioxacin on hepatic genes expression of P450 isoforms (CYP1A and CYP3A), GST and P-glycoprotein (P-gp) in Swordtail fish (Xiphophorus Helleri)
文献类型: 外文期刊
作者: Liang, Ximei 1 ; Wang, Lan 1 ; Ou, Ruikang 1 ; Nie, Xiangping 1 ; Yang, YuFeng 1 ; Wang, Fang 3 ; Li, Kaibin 3 ;
作者机构: 1.Jinan Univ, Dept Ecol, Guangzhou 510632, Guangdong, Peoples R China
2.Minister Educ, Res Ctr Trop & Subtrop Aquat Ecol Engn, Guangzhou 510632, Guangdong, Peoples R China
3.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China
关键词: Norfloxacin;Swordtail fish (Xiphophorus helleri);Sub-chronic toxicity;Enzyme activity;Gene expression
期刊名称:ECOTOXICOLOGY ( 影响因子:2.823; 五年影响因子:2.958 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: The presence of antibiotics including norfloxacin in the aquatic environment may cause adverse effects in non-target organisms. But the toxic mechanisms of fluoroquinolone to fish species are still not completely elucidated. Thus, it is essential to investigate the response of fish to the exposure of fluoroquinolone at molecular or cellular level for better and earlier prediction of these environmental pollutants toxicity. The sub-chronic toxic effects of norfloxacin (NOR) on swordtail fish (Xiphophorus helleri) were investigated by measuring mRNA expression of cytochrome P450 1A (CYP1A), cytochrome P450 3A (CYP3A), glutathione S-transferase (GST) and P-glycoprotein (P-gp) and their corresponding enzyme activities (including ethoxyresorufin O-deethylase, erythromycin N-demethylase and GST. Results showed that NOR significantly affected the expression of CYP1A, CYP3A, GST and P-gp genes in swordtails. The gene expressions were more responsive to NOR exposure than their corresponding enzyme activities. Moreover, sexual differences were found in gene expression and enzyme activities of swordtails exposed to NOR. Females displayed more dramatic changes than males. The study further demonstrated that the combined biochemical and molecular parameters were considered as useful biomarkers to improve our understanding of potential ecotoxicological risks of NOR exposure to aquatic organisms.
- 相关文献
作者其他论文 更多>>
-
Construction of P450 scaffold biocatalysts for the biodegradation of five chloroanilines
作者:Yang, Ridong;Wang, Fang;Xu, Jiakun;Yang, Ridong;Che, Hongxia;Shoji, Osami;Lin, Yingwu
关键词:P450BM3; Artificial metalloenzyme; Chloroanilines; Biodegradation
-
Construction of artificial peroxidase based on myoglobin scaffold for efficient degradation of meloxicam
作者:Zhang, Weikang;Yang, Yadan;Wang, Fang;Xu, Jiakun;Zhang, Weikang;Yang, Yadan;Meng, Xiangmin;Lin, Ying-Wu
关键词:Meloxicam; Myoglobin; Biodegradation; Structure-function relationship; Degradation; Environmental remediation
-
Regioselective aromatic O-demethylation with an artificial P450BM3/sugar alcohol oxidase peroxygenase system
作者:Zhao, Sijia;Wang, Jinghan;Li, Dong;Wang, Fang;Xu, Jiakun;Zhao, Sijia;Wang, Jinghan;Shoji, Osami;Lin, Yingwu
关键词:Lignin; P450BM3; O-demethylation
-
Metabolic potentials of the gut microbes in Antarctic krill (Euphausia superba)
作者:Wei, Zhanfei;Du, Sheng;Liu, Kaiqiang;Shao, Changwei;Meng, Lihui;Wang, Fang;Wang, Xinliang;Xu, Qingchang;Mu, Xiuxia;Jiang, Aijun;Lu, Rui;Meng, Liang
关键词:
Euphausia superba ; gut microbial diversity; metabolic function; direct metagenomic; culture-enriched metagenomics -
A chromosome-level genome assembly of the male darkbarbel catfish (Pelteobagrus vachelli) using PacBio HiFi and Hi-C data
作者:Liu, Haiyang;Zhang, Jin;Cui, Tongxin;Zhang, Xincheng;Li, Kaibin;Wang, Fang;Luo, Qing;Fei, Shuzhan;Chen, Kunci;Zhu, Xinping;Zhao, Jian;Ou, Mi;Zhang, Jin;Zhao, Jian;Cui, Tongxin;Zhao, Jian;Chen, Baixiang;Zhu, Chunkun;Li, Bingjie;Fang, Lingzhao
关键词:
-
Trends in Fishery Ecosystem Stability in the East China Sea under Dual Pressures of Fishing and Global Warming
作者:Yang, Qian;Cheng, Heqin;Li, Zhenqi;Ren, Zhongda;Ding, Weijie;Wu, Junhao;Jiang, Hong;Yang, Qian;Shi, Xuming;Wang, Fang;Shi, Xuming;Wang, Fang;Luo, Yusen;Yang, Qian;Cheng, Heqin;Han, Zhen;Fan, Wei;Ren, Zhongda
关键词:global warming; fishery; East China Sea; fisheries ecosystems; sustainable development; climate adaptation strategies
-
Transcriptomics and metabolomics reveal the regulation of reproductive performance and egg quality of blotched snakehead Channa maculata broodstock induced by a high protein diet
作者:Fei, Shuzhan;Ou, Mi;Liu, Haiyang;Zhang, Xincheng;Luo, Qing;Li, Kaibin;Chen, Kunci;Zhao, Jian;Chen, Baixiang
关键词:Blotched snakehead; Dietary protein; Egg quality; Sex steroid hormone; Reproductive performance



