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Suppressive Effects of Barley beta-Glucans with Different Molecular Weight on 3T3-L1 Adipocyte Differentiation

文献类型： 外文期刊

作者： Zhu, Yingying 1 ; Yao, Yang 1 ; Gao, Yue 1 ; Hu, Yibo 1 ; Shi, Zhenxing 1 ; Ren, Guixing 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China

2.Univ Liege, Gembloux Agrobio Tech, Anim Sci Unit, Passage Deportes 2, B-5030 Gembloux, Belgium

3.Western Univ, Qilu Univ Technol, Daxue Rd,Sci Pk, Jinan 250353, Shandong, Peoples R China

关键词： barley beta-glucans;3T3-L1 cells;PPAR gamma;C/EBP alpha

期刊名称：JOURNAL OF FOOD SCIENCE （ 影响因子：3.167； 五年影响因子：3.376 ）

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收录情况： SCI

摘要： In this study, 2 beta-glucans with different molecular weight were prepared and purified from hull-less barley bran. The aim was to evaluate their effects on the differentiation of 3T3-L1 pre-adipocytes. Results showed that barley beta-glucans inhibited the differentiation of 3T3-L1 pre-adipocytes induced by differentiation medium in a dose-dependent manner, the suppressive effect of high-molecular-weight barley beta-glucans (552 kDa, BGH) was stronger (P < 0.05) than that of low-molecular-weight barley beta-glucan (32 kDa, BGL), evidenced by the significantly decrease (P < 0.05) of Oil-red O staining and intracellular triglyceride content in the mature adipocytes. Besides, gene expression analysis and Western Blot analysis revealed that both BGH and BGL inhibited the mRNA and protein levels of adipogenesis related transcription factors peroxisome proliferator-activated receptor. (PPAR.) and CCAAT-enhancer-binding protein a (C/EBP alpha) which are principal regulators of adipogenesis. Furthermore, the mRNA and protein expression levels of PPAR. target genes in adipose tissue including adipocyte fatty acid binding protein (ap2), lipoprotein lipase (LPL), uncoupling protein-2 (UCP-2), and glucose-transporter 4 (Glut4) in 3T3-L1 cells was also markedly downregulated (P < 0.05). These findings were anticipated to help develop barley beta-glucans based functional food for the management of obesity.

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