TWO GENES WITH FERTILE ATTRIBUTES FROM MACROBRACHIUM NIPPONENSE (DE HAAN, 1849) (NATANTIA: PALAEMONIDAE): EVIDENCE FROM EXPRESSION ANALYSIS OF MAGO NASHI AND TSUNAGI PROTEINS DURING OOCYTE MATURATION AND EMBRYONIC DEVELOPMENT
文献类型: 外文期刊
作者: Zhang, Fengying 1 ; Chen, Liqiao 1 ; Zhao, Weihong 1 ; Wu, Ping 1 ; Li, Erchao 1 ; Yu, Na 1 ; Qin, Jianguang 3 ; Ma, Ling 1 ;
作者机构: 1.E China Normal Univ, Coll Life Sci, 3663 North Zhongshan Rd, Shanghai 200062, Peoples R China
2.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, 300 Jungong Rd, Shanghai 200090, Peoples R China
3.Flinders Univ S Australia, Sch Biol Sci, GPO Box 2100, Adelaide, SA 5001, Australia
关键词: aquaculture;DNA;larvae;reproductive physiology;RNA
期刊名称:JOURNAL OF CRUSTACEAN BIOLOGY ( 影响因子:1.43; 五年影响因子:1.501 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Mago nashi (Mago) and Tsunagi (Y14) are the core components of the exon junction complex that play important roles in RNA metabolism and development of eukaryotic organisms. We identified two cDNAs encoding Mago and Tsunagi protein from the oriental river prawn Macrobrachium nipponense (De Haan, 1849) and termed MnMago and MnTsu, respectively. The MnMago cDNAs encoded a 147 amino-acid protein with an obvious "Mago nashi" domain. The MnTsu cDNA encoded a 161-amino-acid protein with a conserved RNA recognition motif. Prediction of interaction modeling showed that Mago and Tsunagi proteins formed a Mago-Tsunagi dimer complex, indicating that the two genes may play a biological function through the Mago-Tsunagi protein complex in M. nipponense. Real-time quantitative PCR analyses showed that the expressions of MnMago and MnTsu in the ovary were consistent among developmental stages. The expression gradually increased from perinucleolus- to oil-globule stage, and reached the peak at the secondary vitellogenesis stage. After a sharp decrease at oocyte maturation stage, the expressions bounced back at the paracmasis stage. The expression patterns of the two transcripts were also synchronized with embryonic development. The highest expression appeared at the protozoea stage, suggesting that MnMago and MnTsu play an important role in the process of embryogenesis. The Mago and Tsunagi cDNA sequences were sequenced from the oriental river prawn for the first time and the pattern of gene expressions was described during oocyte maturation and embryonic development. This study indicates that these two genes in the embryo and ovary perform important roles in reproductive physiology in the oriental river prawn.
- 相关文献
作者其他论文 更多>>
-
Effects of estradiol on fatty acid composition and tissue structure of hepatopancreas in Procambarus clarkii
作者:Zhao, Weihong;Liu, Jintao;Zheng, Xirui;Lv, Linlan;Chen, Jiyi;Wang, Jiamei;Sui, Yanming;Zhao, Weihong;Zheng, Liang
关键词:Procambarus clarkii; Estrogen; Fatty acid; Hepatopancreas; Histology
-
Temporal and Spatial Signatures of Scylla paramamosain Transcriptome Reveal Mechanistic Insights into Endogenous Ovarian Maturation under Risk of Starvation
作者:Fu, Yin;Zhang, Fengying;Wang, Wei;Zhao, Ming;Ma, Chunyan;Chen, Wei;Liu, Zhiqiang;Ma, Keyi;Ma, Lingbo;Fu, Yin;Cheng, Yongxu;Xu, Jiayuan;Su, Zhixing;Lv, Xiaokang
关键词:Scylla paramamosain; ovarian development; starvation; autophagy; gene expression; biochemical analysis
-
Feeding rhythm of the zoea larvae of Scylla paramamosain: The dynamic feeding rhythm is not completely synchronized with photoperiod
作者:Wang, Wei;Liu, Zhiqiang;Wang, Xueyang;Zhang, Fengying;Ma, Chunyan;Zhao, Ming;Ma, Keyi;Ma, Lingbo
关键词:Scylla paramamosain; Zoea; Feeding rhythm; Feeding rate; Digestive enzyme
-
Spatial Genetic Structure and Diversity of Large Yellow Croaker (Larimichthys crocea) from the Southern Yellow Sea and North-Central East China Sea: Implications for Conservation and Stock Enhancement
作者:Zhang, Fengying;Jiang, Yazhou;Ma, Chunyan;Chen, Wei;Cheng, Jiahua;Ma, Lingbo
关键词:Larimichthys crocea; mitochondrial COI; genetic diversity; population; conservation
-
Transcriptome and molecular regulatory mechanisms analysis of gills in the black tiger shrimp Penaeus monodon under chronic low-salinity stress
作者:Li, Yun-Dong;Li, ErChao;Li, Yun-Dong;Si, Meng-Ru;Jiang, Shi-Gui;Jiang, Song;Yang, Li-Shi;Huang, Jian-Hua;Zhou, Fa-Lin;Li, Yun-Dong;Yang, Qi-Bin;Chen, Xu;Zhou, Fa-Lin;Li, Yun-Dong;Zhou, Fa-Lin;Li, ErChao
关键词:Penaeus Monodon; Chronic low-salinity stress; transcriptome; osmoregulation; adaptation mechanisms
-
Molecular characterization and transcriptional response to TiO2-GO nanomaterial exposure of two molt-related genes in the juvenile prawn, Macrobrachium rosenbergii
作者:Guo, Ziqi;Xu, Likun;Wang, Wei;Chen, Wei;Ma, Chunyan;Zhang, Fengying;Ma, Lingbo;Liu, Zhiqiang;Ma, Keyi;Guo, Ziqi
关键词:
-
Effects of four diets on the metabolism of megalopa metamorphosis of the mud crab, Scylla paramamosain
作者:Ma, Keyi;Liu, Zhiqiang;Qiao, Guangde;Ma, Lingbo;Zhang, Fengying;Zhao, Ming;Ma, Chunyan;Wang, Wei
关键词:crustacean; Scylla paramamosain; megalopa; metamorphosis; metabolomics; fatty acid content