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IDENTIFICATION OF miRNAs IN SWEET POTATO BY SOLEXA SEQUENCING

文献类型: 外文期刊

作者: Bian, X. 1 ; E, Z. 2 ; Ma, P. 1 ; Jia, Z. 1 ; Guo, X. 1 ; Xie, Y. 1 ;

作者机构: 1.Jiangsu Acad Agr Sci, Prov Key Lab Agrobiol, Inst Food Crops, Nanjing, Jiangsu, Peoples R China

2.China Natl Rice Res Inst, Hangzhou, Zhejiang, Peoples R China

关键词: Ipomoea batatas;microRNAs;deep sequencing;conserved miRNA;target

期刊名称:RUSSIAN JOURNAL OF PLANT PHYSIOLOGY ( 影响因子:1.481; 五年影响因子:1.608 )

ISSN:

年卷期:

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收录情况: SCI

摘要: MicroRNAs (miRNAs) are small endogenous RNAs, involved in plant growth and development as well as in stress responses. Among them, some are highly evolutionally conserved in the plant kingdom, which provides a powerful strategy for identifying miRNAsin a new species. Sweet potato (Ipomoea batatas L.) is one of the important food crops in the world, but few of its miRNAs have been determined. In this study, a total of 24 conserved miRNAs belonged to 14 miRNA families, and 16 novel miRNAs were identified by deep sequencing. Using previously established protocols, a total of 48 potential target genes were predicted for the conserved and novel miRNAs. These target genes are involved in transcription, metabolism, defense response, oxidation-reduction processes, etc. Overall, this study provides information concerning the miRNAs precursors in /. batatas, mature miRNAs, and miRNA targets, and it is valuable as the basis for the future research of miRNA functions in I. batatas.

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