您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Cloning of Litopenaeus vannamei CD63 and it's role in white spot syndrome virus infection

文献类型： 外文期刊

作者： Guan, Guang-Kuo 1 ; Liu, Qing-Hui 1 ; Li, Chen 1 ; Huang, Jie 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China

2.Shanghai Ocean Univ, Shanghai, Peoples R China

3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Aquaculture, Qingdao, Peoples R China

关键词： Litopenaeus vannamei;CD63;Cloning;WSSV;Interaction

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： White Spot Syndrome Virus (WSSV) is currently the most serious shrimp pathogen, which has brought huge losses to shrimp industry worldwide. CD63 of shrimp belongs to the tetraspanin superfamily, which plays an important role in signal transduction and immune process. In this paper, CD63 cDNA sequence of Litopenaeus vannamei was cloned using RACE method. The amplified sequence is 1472 bp, with its ORF 744 bp, encoding 247 amino acids. Bioinformatics analysis showed that the sequence of LvCD63 has 93% similarity with Penaeus monodon and 92% similarity with Fenneropenaeus chinensis. Real-time PCR analysis showed that the mRNA levels of LvCD63 expressed in the tissues of hemocytes, gill, epithelial tissue, heart, lymphoid, hepatopancreas, stomach, intestines, muscle and nerve. Among these tissues the highest expression level was showed in the tissue of haemolymph, followed by epithelial tissue, hepatopancreas, and nerve. The lowest expression level of LvCD63 was appeared in the muscle tissue. After WSSV challenge, the expression levels of LvCD63 were both up-regulated in the tissues of gill and epithelial. However the expression level of LvCD63 in hepatopancreas was down-regulated. Far-western blot analysis showed that LvCD63 interacts with VP28, and both VP28N and VP28C fragments interact with LvCD63. Flow cytometry analysis showed that LvCD63 was present on the surface of hemocytes and it is required for binding of WSSV virions. Neutral experiments in vivo showed that LvCD63LEL delayed WSSV infection in shrimp. (C) 2016 Elsevier Ltd. All rights reserved.

  • 相关文献

[1]Litopenaeus vannamei clathrin coat AP17 involved in white spot syndrome virus infection. Wang, Xiu-Fang,Liu, Qing-Hui,Huang, Jie,Wang, Xiu-Fang,Wu, Yin,Liu, Qing-Hui,Huang, Jie.

[2]Efficiency of two fragments of VP28 against White Spot Syndrome Virus in Litopenaeus vannamei. Qiu, Zhi-guang,Liu, Qing-hui,Huang, Jie. 2012

[3]Comparison of White Spot Syndrome Virus Infection Resistance Between Exopalaemon carinicauda and Litopenaeus vannamei Under Different Salinity Stresses. Ge Qianqian,Yu Ge,Sun Ming,Li Jitao,Li Jian,Ge Qianqian,Li Jitao,Li Jian. 2017

[4]White spot syndrome virus VP51 interact with ribosomal protein L7 of Litopenaeus vannamei. Liu, Qing-hui,Ma, Fang-fang,Guan, Guang-Kuo,Wang, Xiu-Fang,Li, Chen,Huang, Jie,Liu, Qing-hui,Li, Chen,Huang, Jie,Ma, Fang-fang,Guan, Guang-Kuo,Wang, Xiu-Fang.

[5]Thioredoxin of Litopenaeus vannamei facilitated white spot syndrome virus infection. Liu, Peng-fei,Liu, Qing-hui,Huang, Jie,Liu, Qing-hui,Huang, Jie,Liu, Peng-fei,Wu, Yin.

[6]Studies of the viral binding proteins of shrimp BP53, a receptor of white spot syndrome virus. Li, Chen,Gao, Xiao-Xiao,Huang, Jie,Liang, Yan.

[7]Molecular cloning and expression analysis of Relish gene from the ridgetail white prawn Exopalaemon carinicauda. Ge, Qianqian,Ge, Qianqian,Li, Jitao,Li, Jian,Zhao, Fazhen,Ren, Hai,Liang, Junping,Duan, Yafei.

[8]The interaction of salinity and Na/K ratio in seawater on growth, nutrient retention and food conversion of juvenile Litopenaeus vannamei. Zhu, CB,Dong, SL,Wang, F. 2006

[9]溶藻弧菌用于对虾益生菌的效果评价. 宋晓玲. 2009

[10]不同饵料组合对中国对虾幼体WSSV携带量及存活率的影响. 肖广侠,孟宪红,孔杰,曹宝祥,刘宁,张庆文,罗坤,李战军. 2013

[11]中国对虾抗病性状遗传标记筛选及遗传多样性分析. 李素红,张天时,孔杰,孟宪红. 2009

[12]白斑综合征病毒感染凡纳滨对虾(Litopenaeus vannamei)TRx、LvP38、CAT、POD基因的表达. 刘鹏飞,刘庆慧,吴垠,黄倢. 2015

[13]白斑综合征病毒2014年中国毒株变异区的序列比较. 孙新颖,万晓嫒,刘庆慧,黄倢. 2016

[14]检测对虾白斑综合症病毒(WSSV)与宿主细胞结合的荧光素标记法. 冯书营,黄倢,张仕璀. 2004

[15]2013年中国典型对虾养殖区白斑综合征病毒流行株高变异区序列的分析比较. 孙新颖,万晓媛,刘庆慧,黄倢. 2016

[16]人工培育对虾苗种体内可培养细菌数量及组成分析. 张晓静,宋晓玲,万晓媛,杨冰,黄倢. 2016

[17]聚β-羟基丁酸酯对凡纳滨对虾抗WSSV能力及免疫基因表达量的影响. 邓康裕,孔杰,孟宪红,罗坤,栾生,曹宝祥,刘宁. 2015

[18]饲料中添加复合芽孢杆菌对凡纳滨对虾抗病毒感染能力及抗病基因表达的影响. 孙博超,杨运楷,李玉宏,宋晓玲,黄倢. 2019

[19]酵母双杂交系统用于WSSV粘附蛋白VP37相关蛋白基因的研究. 张莉,黄倢,戴继勋. 2005

[20]2017年中国部分地区白斑综合征病毒高变异区的序列分析. 刘晓娉,孙新颖,刘庆慧,万晓媛,黄倢. 2019

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台