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Cloning and Transformation of EeHKT1;4 Gene from Elytrigia elongata

文献类型： 外文期刊

作者： Meng, Lin 1 ; Zhang, Lin 1 ; Guo, Qiang 1 ; Li, Shan-Shan 1 ; Mao, Pei-Chun 1 ; Tian, Xiao-Xia 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Res & Dev Ctr Grass & Environm, Beijing 100097, Peoples R China

关键词： Clone;EeHKT1;4 gene;Elytrigia elongata (Host) Nevski;plant expression vector;transformation;transgenic tobacco

期刊名称：PROTEIN AND PEPTIDE LETTERS （ 影响因子：1.89； 五年影响因子：1.528 ）

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收录情况： SCI

摘要： The EeHKT1;4 gene was firstly cloned from Elytrigia elongata by RT-PCR technique with 1977 bp full-length cDNA encoding 1722 bp open reading frame (ORF) and 573 amino acids. The PCR fragment of EeHKT1;4 gene was inserted into the binary vector pBI121 and got the resulted expression vector, which named pBI121-35S-EeHKT1;4-Nos. The vector was further transformed into the agrobacterium EHA105, and then EeHKT1;4 gene was transferred into tobacco by the Agrobaterium-mediated genetic transformation method. The results showed that the target gene was inserted into the genomes of tobacco and expressed. Therefore, the transgenic tobacco (T-0) plants overexpressing EeHKT1;4 gene were successfully obtained in this study. And EeHKT1;4 reduces Na+ concentration in the leaves of T-0 plants, thereby plays a central role in protecting plant leaves from salinity stress.

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