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Molecular characterization and mRNA expression of hypoxia inducible factor-1 and cognate inhibiting factor in Macrobrachium nipponense in response to hypoxia

文献类型： 外文期刊

作者： Sun, Shengming 1 ; Xuan, Fujun; Fu, Hongtuo 1 ; Ge, Xianping 1 ; Zhu, Jian 1 ; Qiao, Hui 1 ; Jin, Shubo 1 ; Zhang, Wen 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Minist Agr, Freshwater Fisheries Res Ctr, Key Lab Genet Breeding & Aquaculture Biol Freshwa, Wuxi 214081, Peoples R China

2.Chinese Acad Fishery Sci, Minist Agr, Freshwater Fisheries Res Ctr, Key Lab Genet Breeding & Aquaculture Biol Freshwa, Wuxi 214081

关键词： Hypoxia;HIF-1;FIH-1;Gene expression;Macrobrachium nipponense

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY （ 影响因子：2.231； 五年影响因子：2.215 ）

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收录情况： SCI

摘要： Hypoxia inducible factors (HIFs) are considered to be the master switches of oxygen-dependent gene expression in mammalian species. Currently, very little is known about the function of this important pathway or the molecular structures of key players in the hypoxia-sensitive Oriental River Prawn Macro brachium nipponense. In this study, HIFs-1 alpha (HIF-1 alpha),-1 beta (HIF-1 beta) and HIF 1-alpha inhibitor (FIH-1) from M. nipponense were cloned. The 4903-bp cDNA of M. nipponense HIF-1 alpha (MnHIF-1 alpha) encodes a protein of 1088 aa, M. nipponense HIF-1 beta (MnHIF-1 beta) spans 2042 bp encoding 663 aa and the 1163 bp M. nipponense NH-1 (MnFIH-1) specifies a polypeptide of 345 aa. MnHIF-1 and MnFIH-1 homologs exhibit significant sequence similarity and share key functional domains with previously described vertebrate and invertebrate isoforms. Phylogenetic analysis identifies that genetic diversification of HIF-1 and FIH-1 occurred within the invertebrate lineage, indicating functional specialization of the oxygen sensing pathways in this group. Quantitative real-time RT-PCR demonstrated that MnHIF-1 and MnFIH-1 mRNA are expressed in different tissues and exhibit transcriptional responses to severe hypoxia in gill and muscle tissue, consistent with their putative role in oxygen sensing and the adaptive response to hypoxia. The role of HIF-1 alpha in response to hypoxia was further investigated in the gills and muscles of prawns using in situ hybridization. These results suggested that HIF-1 alpha plays an important role in oxygen sensing and homeostasis in M. nipponense. (C) 2016 Elsevier Inc. All rights reserved.

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[1]Molecular Cloning and Functional Characterization of a Hexokinase from the Oriental River Prawn Macrobrachium nipponense in Response to Hypoxia. Sun, Shengming,Fu, Hongtuo,Zhu, Jian,Ge, Xianping,Xuan, Fujun. 2017

[2]Transciptomic and histological analysis of hepatopancreas, muscle and gill tissues of oriental river prawn (Macrobrachium nipponense) in response to chronic hypoxia. Sun, Shengming,Fu, Hongtuo,Zhu, Jian,Ge, Xianping,Xuan, Fujun,Gu, Zhimin. 2015

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[7]THE METALLOTHIONEIN GENE FROM THE ORIENTAL RIVER PRAWN MACROBRACHIUM NIPPONENSE (DE HAAN, 1849): CHARACTERIZATION AND EXPRESSION IN RESPONSE TO HYPDXIA AND REOXYGENATION. Sun, Shengming,Gu, Zhimin,Fu, Hongtuo,Zhu, Jian.

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