您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Transcriptome analysis of 'Sijihua' longan (Dimocarpus longan L.) based on next-generation sequencing technology

文献类型： 外文期刊

作者： Zhang, H. N. 1 ; Shi, S. Y. 1 ; Li, W. C. 1 ; Shu, B. 1 ; Liu, L. Q. 1 ; Xie, J. H. 1 ; Wei, Y. Z. 2 ;

作者机构： 1.Chinese Acad Trop Agr Sci, South Subtrop Crops Res Inst, Minist Agr, Key Lab Trop Fruit Biol, 1 Huxiu Rd, Zhanjiang 524091, Guangdong, Peoples R China

2.Chinese Acad Trop Agr Sci, South Subtrop Crops Res Inst, Minist Agr, Key Lab Trop Fruit Biol, 1 Huxiu Rd, Zhanji

关键词： 'Sijihua' longan;Dimocarpus longan Lour;transcriptome;flowering

期刊名称：JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY （ 影响因子：1.641； 五年影响因子：1.616 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： 'Sijihua' longan (Dimocarpus longan Lour.) blossoms throughout the year, and hence it is a useful material for studies on the mechanism of flowering. However, few genomic resources such as DNA sequences or markers are currently available for 'Sijihua' longan. To increase our understanding of the genetic control of important flowering characteristics in 'Sijihua' longan, we constructed a mixed cDNA library of 'Sijihua' longan using Illumina paired-end sequencing technology and obtained a total of 90,069,246 high-quality reads. Among the unigenes, 43.16% (59,588) had at least one BLASTX hit against the Arabidopsis thaliana sequence database and 42.83% (59,130) had significant similarity with proteins in the NCBI non-redundant protein database. Of these annotated unigenes, a total of 15,956 (11.56%) were mapped onto 271 pathways using the Kyoto Encyclopedia of Genes and Genomes Pathway database. Furthermore, 11,402 unigenes were converted into 13,236 potential simple sequence repeats (SSRs). Dinucleotide SSRs were the dominant repeat motif, with a frequency of 54.3%, followed by trinucleotide (34.6%) and tetranucleotide (4.7%) repeat motifs. AG/CT repeats (46.29%) were the most frequent motifs in the data set. We focused on gene discovery in the area of regulation of flowering, and transcripts were identified for almost every gene involved in the control of flowering. This study demonstrates that Illumina sequencing technology is a rapid and informative approach to gene discovery and molecular marker development in non-model organisms without a whole genome database. Our results provide a comprehensive sequence resource for investigating the mechanisms of flowering in 'Sijihua' longan, and will establish a platform for further studies on longan.

  • 相关文献

[1]Transcriptome Sequencing Determined Flowering Pathway Genes in Aechmea fasciata Treated with Ethylene. Li, Zhiying,Wang, Jiabin,Zhang, Xuequan,Lei, Ming,Fu, Yunliu,Zhang, Jing,Wang, Zhi,Xu, Li,Li, Zhiying,Wang, Jiabin,Zhang, Xuequan,Lei, Ming,Fu, Yunliu,Zhang, Jing,Wang, Zhi,Xu, Li.

[2]Molecular Cloning and Characterization of Four Genes Encoding Ethylene Receptors Associated with Pineapple (Ananas comosus L.) Flowering. Li, Yun-He,Wu, Qing-Song,Liu, Sheng-Hui,Zhang, Hong-Na,Zhang, Zhi,Sun, Guang-Ming,Li, Yun-He,Huang, Xia. 2016

[3]Exogenous gibberellic acid increases the fruit weight of 'Comte de Paris' pineapple by enlarging flesh cells without negative effects on fruit quality. Li, Yun-He,Zou, Ming-Hong,Zhang, Zhi,Sun, Guang-Ming,Li, Yun-He,Sun, Guang-Ming,Wu, Yong-Jie,Wu, Bei.

[4]AfAP2-1, An Age-Dependent Gene of Aechmea fasciata, Responds to Exogenous Ethylene Treatment. Lei, Ming,Li, Zhi-Ying,Wang, Jia-Bin,Fu, Yun-Liu,Ao, Meng-Fei,Xu, Li. 2016

[5]Molecular Cloning, Characterization, and Expression of MiSOC1: A Homolog of the Flowering Gene SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 from Mango (Mangifera indica L). Wei, Junya,Liu, Debing,Liu, Guoyin,Tang, Jie,Wei, Junya,Chen, Yeyuan. 2016

[6]Identification, Functional Study, and Promoter Analysis of HbMFT1, a Homolog of MFT from Rubber Tree (Hevea brasiliensis). Bi, Zhenghong,Bi, Zhenghong,Huang, Huasun,Hua, Yuwei,Li, Xiang. 2016

[7]De novo assembly and characterization of bark transcriptome using Illumina sequencing and development of EST-SSR markers in rubber tree (Hevea brasiliensis Muell. Arg.). Li, Dejun,Deng, Zhi,Qin, Bi,Liu, Xianghong,Men, Zhonghua. 2012

[8]De Novo Assembly and Characterization of Pericarp Transcriptome and Identification of Candidate Genes Mediating Fruit Cracking in Litchi chinensis Sonn.. Li, Wei-Cai,Zhang, Hong-Na,Shi, Sheng-You,Liu, Li-Qin,Shu, Bo,Liang, Qing-Zhi,Xie, Jiang-Hui,Wei, Yong-Zan,Wu, Jian-Yang. 2014

[9]Transcriptome characterization and expression profiles of the related defense genes in postharvest mango fruit against Colletotrichum gloeosporioides. Hong, Keqian,Gong, Deqiang,Zhang, Lubin,Hu, Huigang,Jia, Zhiwei,Gu, Hui,Song, Kanghua. 2016

[10]Transcriptome Profiling of Light-Regulated Anthocyanin Biosynthesis in the Pericarp of Litch. Zhang, Hong-Na,Li, Wei-Cai,Shi, Sheng-You,Shu, Bo,Liu, Li-Qin,Wei, Yong-Zan,Xie, Jiang-Hui,Wang, Hui-Cong. 2016

[11]De Novo Analysis of the Transcriptome of Meloidogyne enterolobii to Uncover Potential Target Genes for Biological Control. Li, Xiangyang,Yang, Dan,Zhao, Jianlong,Jian, Heng,Li, Xiangyang,Niu, Junhai,Niu, Junhai. 2016

[12]Comparative transcriptome analysis of latex from rubber tree clone CATAS8-79 and PR107 reveals new cues for the regulation of latex regeneration and duration of latex flow. Chao, Jinquan,Chen, Yueyi,Wu, Shaohua,Tian, Wei-Min. 2015

[13]Identification of novel microRNAs in Hevea brasiliensis and computational prediction of their targets. Gebelin, Virginie,Argout, Xavier,Engchuan, Worrawat,Pitollat, Bertrand,Duan, Cuifang,Montoro, Pascal,Leclercq, Julie,Engchuan, Worrawat,Duan, Cuifang. 2012

[14]Transcriptome sequencing and analysis of rubber tree (Hevea brasiliensis Muell.) to discover putative genes associated with tapping panel dryness (TPD). Liu, Jin-Ping,Tian, Xiao-Yan,Xia, Zhi-Qiang,Li, Yi-Jian. 2015

[15]Development of gene-based simple sequence repeat markers for association analysis in Cocos nucifera. Xia, Wei,Xiao, Yong,Liu, Zheng,Luo, Yi,Fan, Haikuo,Yang, Yaodong,Zhao, Songlin,Mason, Annaliese S.,Fan, Haikuo,Peng, Ming. 2014

[16]Transcriptomic identification of chemoreceptor genes in the red palm weevil Rhynchophorus ferrugineus. Yan, W.,Liu, L.,Qin, W. Q.,Li, C. X.,Yan, W.,Peng, Z. Q.. 2015

[17]Identification of the Hevea brasiliensis AP2/ERF superfamily by RNA sequencing. Duan, Cuifang,Argout, Xavier,Gebelin, Virginie,Summo, Marilyne,Dufayard, Jean-Francois,Leclercq, Julie,Piyatrakul, Piyanuch,Pirrello, Julien,Rio, Maryannick,Montoro, Pascal,Duan, Cuifang,Kuswanhadi,Piyatrakul, Piyanuch,Champion, Antony. 2013

[18]Comparative Transcriptome Analysis of Latex Reveals Molecular Mechanisms Underlying Increased Rubber Yield in Hevea brasiliensis Self-Rooting Juvenile Clones. Li, Hui-Liang,Guo, Dong,Zhu, Jia-Hong,Wang, Ying,Chen, Xiong-Ting,Peng, Shi-Qing. 2016

[19]Transcription Profiling Analysis of Mango-Fusarium Mangiferae Interaction. Liu, Feng,Wu, Jing-bo,Zhan, Ru-lin,Ou, Xiong-chang. 2016

[20]Transcriptome profiling of litchi leaves in response to low temperature reveals candidate regulatory genes and key metabolic events during floral induction. Zhang, Hongna,Shen, Jiyuan,Chen, Houbin,Zhang, Hongna,Wei, Yongzan. 2017

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台