中国水产科学研究院机构知识库

Cloning and characterization of two duplicated interleukin-17A/F2 genes in common carp (Cyprinus carpio L.): Transcripts expression and bioactivity of recombinant IL-17A/F2

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文献类型：外文期刊

作者： Li, Hongxia ¹ ; Yu, Juhua ¹ ; Li, Jianlin ¹ ; Tang, Yongkai ¹ ; Yu, Fan ¹ ; Zhou, Jie ² ; Yu, Wenjuan ² ;

作者机构： 1.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Resource, Minist Agr, Wuxi 214081, Peoples R China

2.Nanjing Agr Univ, Wuxi Fishery Coll, Wuxi 214081, Peoples R China

关键词： IL-17A/F2;Common carp (Cyprinus carpio);Proinflammatory and innate immune function;Duplicated genes;Expression;Promoter activity

期刊名称：FISH & SHELLFISH IMMUNOLOGY （影响因子：4.581；五年影响因子：4.851 ）

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收录情况： SCI

摘要： Interleukin-17 (IL-17) plays an important role in inflammation and host defense in mammals. In this study, we identified two duplicated IL-17A/F2 genes in the common carp (Cyprinus carpio) (ccIL-17A/F2a and ccIL-17A/F2b), putative encoded proteins contain 140 amino acids (aa) with conserved IL-17 family motifs. Expression analysis revealed high constitutive expression of ccIL-17A/F2s in mucosal tissues, including gill, skin and intestine, their expression could be induced by Aeromonas hydrophila, suggesting a potential role in mucosal immunity. Recombinant ccIL-17A/F2a protein (rcclL-17A/F2a) produced in Escherichia coli could induce the expression of proinflammatory cytokines (IL-1 beta) and the antimicrobial peptides S100A1, S100A10a and S100A10b in the primary kidney in.a dose- and time-dependent manner. Above findings suggest that ccIL-17A/F2 plays an important role in both proinflammatory and innate immunity. Two duplicated ccIL-17A/F2s showed different expression level with ccIL-17A/F2a higher than b, comparison of two 5' regulatory regions indicated the length from anticipated promoter to transcriptional start site (TSS) and putative transcription factor binding site (TFBS) were different. Promoter activity of ccIL-17A/F2a was 2.5 times of ccIL-17A/F2b which consistent with expression results of two genes. These suggest mutations in 5'regulatory region contributed to the differentiation of duplicated genes. To our knowledge, this is the first report to analyze 5'regulatory region of piscine IL-17 family genes. (C) 2016 Elsevier Ltd. All rights reserved.

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意见箱

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H2O2-Induced Oxidative Stress Responses in Eriocheir sinensis: Antioxidant Defense and Immune Gene Expression Dynamics

意 见 箱

作者其他论文更多>>

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意见箱