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Cellular responses of the tiger shrimp Penaeus monodon haemocytes after lipopolysaccharide injection

文献类型： 外文期刊

作者： Xian, Jian-An 1 ; Zhang, Xiu-Xia 1 ; Guo, Hui 3 ; Wang, Dong-Mei 1 ; Wang, An-Li 2 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Peoples R China

2.S China Normal Univ, Key Lab Ecol & Environm Sci Guangdong Higher Educ, Guangdong Prov Key Lab Hlth & Safe Aquaculture, Sch Life Sci, Guangzhou 510631, Guangdong, Peoples R China

3.Guangdong Ocean Univ, Coll Fisheries, Key Lab Aquaculture South China Sea Aquat Econ An, Guangdong Higher Educ Inst, Zhanjiang 524025, Peoples R China

关键词： Cellular response;Lipopolysaccharide;Penaeus monodon;Haemocyte;Flow cytometry;Immunity

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

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收录情况： SCI

摘要： This study was aimed at investigating the in vivo effects of lipopolysaccharide (LPS) injection on Penaeus monodon haemocytes at a cellular level. Cellular responses of LPS-injected shrimp were analysed using flow cytometry. Results showed that LPS injection caused total haemocyte count (THC) and count of large cells (semigranular and granular cells) decline. In LPS-injected shrimp, percentage of large cells decreased at the initial stage, and returned to the original level later. After LPS infection, non-specific esterase activity, reactive oxygen species (ROS) production and nitric oxide (NO) production in haemocytes were significantly induced, while apoptotic cell ratio of haemocytes increased. PO activity in plasma increased in shrimp received LPS at 2 mu g g(-1) after 3-12 h and at 8 mu g g(-1) after 3-6 h, and then returned to the initial levels. These results demonstrated that LPS induced immune responses on haemocytes, including production of ROS and NO, and release of esterase and PO. On the other hand, THC reduction might be due to the ROS/NO-induced apoptosis. Haemocyte apoptosis which would eliminate damaged or weak cells and contribute to haemocyte renewal, may be a defending strategie against pathogens. (C) 2016 Elsevier Ltd. All rights reserved.

  • 相关文献

[1]Various cellular responses of different shrimp haemocyte subpopulations to lipopolysaccharide stimulation. Xian, Jian-An,Zhang, Xiu-Xia,Wang, Dong-Mei,Li, Jun Tao,Zheng, Pei-Hua,Lu, Yao-Peng.

[2]Oxidative burst activity in haemocytes of the freshwater prawn Macrobrachium rosenbergii. Xian, Jian-An,Zhang, Xiu-Xia,Li, Jun-Tao,Wang, Dong-Mei,Wang, An-Li,Zheng, Pei-Hua,Lu, Yao-Peng,Ye, Jian-Min. 2018

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