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Toxicity of Tributyltin in Juvenile Common Carp (Cyprinus Carpio): Physiological Responses, Hepatic Gene Expression, and Stress Protein Profiling

文献类型: 外文期刊

作者: Li, Zhi-Hua 1 ; Zhong, Li-Qiao 1 ; Mu, Wei-Na 1 ; Wu, Yan-Hua 1 ;

作者机构: 1.Chinese Acad Fishery Sci, Key Field Stn Fishery Resource & Environm Upper M, Key Lab Freshwater Biodivers Conservat, Minist Agr,Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China

2.Univ South Bohemia Ceske Budejovice, Fac Fisheries & Protect Waters, South Bohemian Res Ctr Aquaculture & Biodivers Hy, Zatisi 728-2, Vodnany 38925, Czech Republic

3.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei, Peoples R China

4.Southwest Univ

关键词: Tributyltin;Common Carp;Physiological Responses;Gene Expression;Protein Profiling

期刊名称:JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY ( 影响因子:3.642; 五年影响因子:3.222 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: In this study, the effects of tributyltin (TBT) on biochemical parameters (antioxidant responses and Na+-K+-ATPase) in different tissues were investigated by using juvenile common carp (Cyprinus Carpio) as well as growth and ion regulation-related genes expression and stress-related proteins profiling in fish liver. Oxidative stress indices and Na+-K+-ATPase showed tissues-specific responses in fish exposed to different TBT concentrations. All tested genes related to GH/IGF-I axis and ion-regulation were significantly induced in the TBT group with lower concentrations (except for the igfbp3 in 10 g/L) and were inhibited in 20 g/L. In addition, the profiling of two proteins Hsp 70 and MT were increasing in a dose-dependent manner under TBT stress. In short, TBT-induced biochemical and molecular responses in different tissues were reflected in the measured parameters in the test. On the basis of TBT residue levels in the natural environment, more long-term experiments at lower concentrations will be necessary in the future.

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