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Development of a restriction length polymorphism combined with direct PCR technique to differentiate goose and Muscovy duck parvoviruses

文献类型： 外文期刊

作者： Wan, Chun-He 1 ; Chen, Hong-Mei 1 ; Fu, Qiu-Ling 1 ; Shi, Shao-Hua 1 ; Fu, Guang-Hua 1 ; Cheng, Long-Fei 1 ; Chen, Cui 1 ;

作者机构： 1.Fujian Acad Agr Sci, Fujian Anim Dis Control Technol Dev Ctr, Inst Anim Husb & Vet Med, Fuzhou 350013, Peoples R China

2.Fujian Agr & Forestry Univ, Coll Life Sci, Fuzhou 350002, People

关键词： direct PCR;goose parvovirus;muscovy duck parvovirus;restriction fragment length polymorphism

期刊名称：JOURNAL OF VETERINARY MEDICAL SCIENCE （ 2020影响因子：1.267； 五年影响因子：1.348 ）

ISSN：

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收录情况： SCI

摘要： A restriction fragment length polymorphism combined with direct PCR technique to differentiate goose and Muscovy duck parvoviruses (GPV and MDPV) was developed based on comparison of the NS gene of GPV and MDPV. Both GPV and MDPV genomic DNA can be amplified with 641 bp using the specific PCR primers. The PCR fragments can be cut into 463 bp and 178 bp only in the case of MDPV-derived PCR products, whereas the GPV-derived PCR products cannot. The method established in this study can be used to differentiate GPV and MDPV with high specificity and precision, by using a direct PCR kit and QuickCut enzyme, as quickly as conventional PCR.

  • 相关文献

[1]Genomic Characterization of Goose Parvovirus and Muscovy Duck Parvovirus Co-infection in Fujian, China. Wan, Chun-he,Hu, Kai-hui,Wan, Chun-he,Chen, Hong-mei,Fu, Qiu-ling,Fu, Guang-hua,Cheng, Long-fei,Shi, Shao-hua,Huang, Yu. 2015

[2]Phylogenetic analysis of VP1 gene sequences of waterfowl parvoviruses from the Mainland of China revealed genetic diversity and recombination. Wang, Shao,Cheng, Xiao-xia,Chen, Shao-ying,Lin, Feng-qiang,Chen, Shi-long,Zhu, Xiao-li,Wang, Jin-xiang,Huang, Mei-qing,Zheng, Min,Wang, Shao,Cheng, Xiao-xia,Chen, Shao-ying,Lin, Feng-qiang,Chen, Shi-long,Zhu, Xiao-li,Wang, Jin-xiang,Huang, Mei-qing,Zheng, Min.

[3]Genomic and pathogenic analysis of a Muscovy duck parvovirus strain causing short beak and dwarfism syndrome without tongue protrusion. Fu, Qiuling,Huang, Yu,Wan, Chunhe,Fu, Guanghua,Cheng, Longfei,Shi, Shaohua,Chen, Hongmei,Liu, Rongchang,Chen, Zhenhai,Qin, Baomin.

[4]Genetic Characterization of a Potentially Novel Goose Parvovirus Circulating in Muscovy Duck Flocks in Fujian Province, China. Wang, Shao,Cheng, Xiao-Xia,Chen, Shao-Ying,Zhu, Xiao-Li,Chen, Shi-Long,Lin, Feng-Qiang,Li, Zhao-Long,Wang, Shao,Cheng, Xiao-Xia,Chen, Shao-Ying,Chen, Shi-Long,Lin, Feng-Qiang. 2013

[5]Complete Genome Sequence of Goose Parvovirus Isolated from Anser cygnoides in China. Wan, Chun-he,Hu, Kai-hui,Wan, Chun-he,Huang, Yu,Shi, Shao-hua,Fu, Guang-hua,Cheng, Long-fei,Chen, Hong-mei,Fu, Qiu-ling,Chen, Cui-teng. 2016

[6]Evidence for natural recombination in the capsid gene VP2 of Taiwanese goose parvovirus. Wang, Shao,Cheng, Xiaoxia,Chen, Shaoying,Lin, Fengqiang,Chen, Shilong,Zhu, Xiaoli,Wang, Jingxiang,Wang, Shao,Cheng, Xiaoxia,Chen, Shaoying,Lin, Fengqiang,Chen, Shilong,Zhu, Xiaoli,Wang, Jingxiang.

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