Molecular mapping of stripe rust resistance gene YrHu derived from Psathyrostachys huashanica
文献类型: 外文期刊
作者: Ma, D. F. 2 ; Fang, Z. W. 2 ; Yin, J. L. 1 ; Chao, K. X. 1 ; Jing, J. X. 1 ; Li, Q. 1 ; Wang, B. T. 1 ;
作者机构: 1.Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China
2.Yangtze Univ, Hubei Collaborat Innocvt Ctr Grain Ind, Jingzhou 434025, Hubei, Peoples R China
3.Hubei Acad Agr Sci, Hubei Key Lab Food Crop Germplasm & Genet Improve, Wuhan 430064, Hubei, Peoples R China
关键词: Puccinia striiformis f. sp tritici;Psathyrostachys huashanica;Stripe rust resistance;Molecular mapping
期刊名称:MOLECULAR BREEDING ( 影响因子:2.589; 五年影响因子:2.75 )
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收录情况: SCI
摘要: Wheat stripe rust is a destructive disease that affects most wheat-growing areas worldwide. Resistance genes from related species and genera add to the genetic diversity available to wheat breeding programs. The stripe rust-resistant introgression line H9020-17-25-6-4 was developed from a cross of resistant Psathyrostachys huashanica with the susceptible wheat cultivar 7182. H9020-17-25-6-4 is resistant to all existing Chinese stripe rust races, including the three most widely virulent races, CYR32, CYR33, and V26. We attempted to characterize this new line by genomic in situ hybridization (GISH) and genetic analysis. GISH using P. huashanica genomic DNA as a probe indicated that the translocated segment was too small to be detected. Genetic analysis involving F-1, F-2, and F2: 3 materials derived from a cross of Mingxian 169 and H9020-17-25-6-4 indicated that a single dominant gene from H9020-17-25-6-4, temporarily designated YrHu, conferred resistance to CYR29 and CYR33. A genetic map consisting of four simple sequence repeat, two sequence-tagged site (STS), and two sequence-related amplified polymorphism markers was constructed. YrHu was located on the short arm of chromosome 3A and was about 0.7 and 1.5 cM proximal to EST-STS markers BG604577 and BE489244, respectively. Both the gene and the closely linked markers could be used in marker-assisted selection.
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