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Characterization and identification of streptococci from golden pompano in China

文献类型: 外文期刊

作者: Cai, X. H. 1 ; Peng, Y. H. 2 ; Wang, Z. C. 2 ; Huang, T. 3 ; Xiong, X. Y. 2 ; Huang, Y. C. 1 ; Wang, B. 1 ; Xu, L. W.; Wu 1 ;

作者机构: 1.Guangdong Ocean Univ, Guangdong Key Lab Control Dis Aquat Econ Anim, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524088, Peoples R China

2.Guangxi Inst Oceanol, Guangxi Key Lab Marine Biotechnol, Beihai 536000, Peoples R China

3.Guangxi Acad Fishery Sci, Guangxi Key Lab Aquat Gene

关键词: Trachinotus ovatus;Streptococcus;Pathogenicity;Biochemical characterization;Multiplex PCR assay

期刊名称:DISEASES OF AQUATIC ORGANISMS ( 影响因子:1.802; 五年影响因子:2.148 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Streptococcal infections cause significant mortality and high economic losses in the fish farm industry worldwide, including in the culture of golden pompano Trachinotus ovatus L., a species gaining popularity in China. A total of 9 streptococcal strains were isolated from cage-cultured diseased golden pompano in Beihai, Zhanjing, and Shenzhen, China, between 2012 and 2014. Conventional and rapid identification systems were used to determine that the isolates were Streptococcus agalactiae, S. iniae, and S. dysgalactiae subsp. dysgalactiae. All isolates were gram-positive cocci cells in pairs or short-chain, non-motile, catalase negative, alpha or beta hemolytic cocci. The results of multiplex PCR assays and 16S rRNA BLAST analysis also showed that the beta hemolytic strains were S. agalactiae and S. iniae and the alpha hemolytic strain was S. dysgalactiae subsp. dysgalactiae, respectively. Pathogenicity assays revealed that S. agalactiae (lethal dose [LD50]: 6.38 x 10(4) CFU ml(-1)) was more virulent for golden pompano than S. iniae (LD50: 1.47 x 10(7) CFU ml(-1)) and S. dysgalactiae subsp. dysgalactiae (LD50: 2.57 x 10(6) CFU ml(-1)) when they were challenged by intraperiotoneal (i.p.) injection. The results of antibiotic susceptibility showed that all strains were extremely susceptible to cefradine, erythromycin, and cefotaxime but resistant to gentamicin, penicillin G, novobiocin, neomycin, ciprofloxacin, roxithromycin, furazolidone, enrofloxacin, norfloxacin, kanamycin, ampicillin, tetracycline, and vancomycin This is the first report of a phenomenon of golden pompano coinfection with S. agalactiae and S. iniae, which will contribute to the diagnosis and prevention of streptococcicosis.

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