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Molecular cloning, expression analysis and cellular localization of an LFRFamide gene in the cuttlefish Sepiella japonica

文献类型： 外文期刊

作者： Cao, Zi-hao 1 ; Sun, Lian-lian 1 ; Chi, Chang-feng 1 ; Liu, Hui-hui 1 ; Zhou, Li-qing 2 ; Lv, Zhen-ming 1 ; Wu, Chang- 1 ;

作者机构： 1.Zhejiang Ocean Univ, Sch Marine Sci & Technol, Natl Engn Res Ctr Marine Facil Aquaculture, Zhoushan 316022, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minister Agr, Qingdao 266071, Peoples R China

关键词： Neuropeptide;FMRFamide-like peptides;LFRFamide;Cuttlefish;Sepiella japonica

期刊名称：PEPTIDES （ 影响因子：3.75； 五年影响因子：3.389 ）

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收录情况： SCI

摘要： Neuropeptides are important regulators of physiological processes in metazoans, such as feeding, reproduction, and heart activities. In this study, an LFRFamide gene was identified from the cuttlefish Sepiella japonica (designated as SjLFRFamide). The full-length sequence of SjLFRFamide cDNA has 841 bp, and the open reading frame contains 567 bp encoding 188 amino acids, which shared high similarity with precursor SOFaRP2 from Sepia officinalis. The deduced SjLFRFamdie precursor protein contains a signal peptide and four different FLPs (FMRFamide-like peptides): one pentapeptide (TIFRFamide), two hexapeptides (NSLFRFamide and GNLFRFamide) and one heptapeptide (PHTPFRFamide). Multiple sequence alignment showed that SjLFRFamide contains rather conserved mature peptides, which all ended in FRF. The phylo-genetic analysis suggests that SjLFRFamide belongs to the LFRFamide subfamily. The tissue distribution analysis through quantitative real-time PCR method showed that SjLFRFamide mRNA is significantly expressed in the brain, and slight trace are detected in female nidamental gland and accessory nidamental gland. In situ hybridization assay of the brain indicated that SjLFRFamide is transcribed in several different functional lobes, suggesting SjLFRFamide might associate with multiple physiological regulations, such as feeding, chromatophore regulation and reproduction. This is the first study describing LFRFamide in S. japonica, which might have great importance for cuttlefish artificial breeding. (C) 2015 Elsevier Inc. All rights reserved.

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