ALTERATIONS OF DNA METHYLATION IN DIVERSE GRAFTED HYBRID TOMATOES (Solanum lycopersicum L.)
文献类型： 外文期刊
作者： Xu Xincheng 1 ; Ali, Zulfiqar 2 ; Song Weiyi 1 ; Ghori, Nida 2 ; Shao Hongbo 4 ; Ding Jinping 1 ;
作者机构： 1.Shangqiu Normal Univ, Dept Life Sci, Shangqiu 476000, Peoples R China
2.Univ Agr Faisalabad, Dept Genet & Plant Breeding, Faisalabad, Pakistan
3.MNS Univ Agr, Dept Plant Breeding & Genet, Multan, Pakistan
4.Jiangsu Acad Agr Sci, Inst Agrobiotechnol, Nanjing 210014, Peoples R China
5.Chinese Acad Sci, Key Lab Coastal Biol & Bioresources Utilizat, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Peoples R China
关键词： MSAP analysis;restriction digestion;PCR;PAGE;silver staining
期刊名称：PAKISTAN JOURNAL OF AGRICULTURAL SCIENCES （ 影响因子：0.748； 五年影响因子：0.854 ）
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收录情况： SCI
摘要： Tomato is an edible and useful vegetable. Navel rot disease and low light stress are the problem in tomato cultivation. Tomato breeding is a potential cheap solution but takes longer time. Grafting technology provides instant solution like cultivating sensitive tomato (scion) grafted on resistant rootstock on non-optimal environmental conditions. DNA methylation of grafted plants might help to adapt to the adverse conditions and has become a hot point in researches concerning vegetable breeding. In this research, disease-resistant tomato cultivar "1221" was used as rootstock, while disease-sensitive tomato cultivar "Money Maker (MM)" was used as scion. After grafting, changes of genomic global DNA methylation were investigated through MSAP analysis. According to MSAP analysis, alterations of degree of DNA methylation patterns were observed, which might help graft hybrids to adapt in response to the internal and external stimuli.
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