您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Rapid and quantitative detection of citrus huanglongbing bacterium 'Candidatus Liberibacter asiaticus' by real-time fluorescent loop-mediated isothermal amplification assay in China

文献类型： 外文期刊

作者： Wu, Xuhui 1 ; Meng, Chunliang 1 ; Wang, Guihua 1 ; Liu, Yuanzheng 1 ; Zhang, Xin 2 ; Yi, Kexian 2 ; Peng, Jun 2 ;

作者机构： 1.Hainan Univ, Coll Environm & Plant Protect, Haikou 570228, Peoples R China

2.Chinese Acad Trop Agr Sci, Key Lab Monitoring & Control Trop Agr & Forest In, Environm & Plant Protect Inst, Haikou 571101, Hainan, Peoples R China

关键词： Huanglongbing (HLB);Candidatus Liberibacter asiaticus (Las);Quantitative detection;Visual inspection;Real-time fluorescence loop-mediated isothermal amplification (RealAmp)

期刊名称：PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY （ 影响因子：2.747； 五年影响因子：2.388 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Huanglongbing (HLB) is a devastating citrus disease that is associated with bacteria of the species 'Candidatus Liberibacter asiaticus (Las)' in China. All currently popular cultivars are susceptible to HLB, and there are no effective approaches to control the disease once the plants have been infected. Therefore, rapid and easy diagnostic tools are needed for maintaining clean nursery stock and inoculum removal strategies to control HLB. A real-time fluorescence loop-mediated isothermal amplification (RealAmp) targeting16S rDNA was developed for the rapid and quantitative detection of Las in China. The detection sensitivity of the RealAmp assay was approximately 1 pg/mu l template DNA (equal to 2.4 x 10(5) target DNA copies/mu l) and no cross-reaction was observed with other pathogens or virus-free seedlings. Besides the quantitative detection using SYTO-9 fluorescent dye, an improved closed-tube visual inspection technique using SYBR Green I staining was developed for rapid screening of samples and minimize the risk of cross-contamination. The RealAmp assay is an alternative quantitative method, which would be used as a routine detection service for nursery materials in China. (C) 2016 Elsevier Ltd. All rights reserved.

  • 相关文献

[1]Development of a real-time fluorescence loop-mediated isothermal amplification assay for rapid and quantitative detection of Fusarium mangiferae associated with mango malformation. Pu, Jinji,Xie, Yixian,Zhang, He,Zhang, Xin,Qi, Yanxiang,Peng, Jun,Peng, Jun,Peng, Jun.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台