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Deep sequencing of banana bract mosaic virus from flowering ginger (Alpinia purpurata) and development of an immunocapture RT-LAMP detection assay

文献类型： 外文期刊

作者： Zhang, Jingxin 1 ; Borth, Wayne B. 1 ; Lin, Birun 2 ; Dey, Kishore K. 1 ; Melzer, Michael J. 1 ; Shen, Huifang 2 ; Pu, 1 ;

作者机构： 1.Univ Hawaii, Coll Trop Agr & Human Resources, Dept Plant & Environm Protect Sci, Honolulu, HI 96822 USA

2.Guangdong Acad Agr Sci, Inst Plant Protect, Key Lab New Tech Plant Protect Guangdong, Guangzhou, Guangdong, Peoples R China

3.Guangdong Acad Agr Sci, Inst Plant Protect, Key Lab New Tech Plant Protect Guangdong, Guangzhou, Guangdong, Peoples R China;

期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）

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收录情况： SCI

摘要： Banana bract mosaic virus (BBrMV) has never been reported in banana plants in Hawaii. In 2010, however, it was detected in a new host, flowering ginger (Alpinia purpurata). In this study, we characterize the A. purpurata isolate and study its spread in flowering ginger in Hawaii. A laboratory study demonstrated that BBrMV could be transmitted from flowering ginger to its natural host, banana, therefore raising a serious concern about the potential risk to the rapidly growing banana industry of Hawaii. To quickly monitor this virus in the field, we developed a robust immunocapture reverse transcription loop-mediated isothermal amplification (IC-RT-LAMP) assay. Deep sequencing of the BBrMV isolate from A. purpurata revealed a single-stranded RNA virus with a genome of 9,713 nt potentially encoding a polyprotein of 3,124 aa, and another predicted protein, PIPO, in the +2 reading-frame shift. Most of the functional motifs in the Hawaiian isolate were conserved among the genomes of isolates from one found in the Philippines and India. However, the A. purpurata isolate had an amino acid deletion in the Pl protein that was most similar to the Philippine isolate. Phylogenetic analysis of an eastern Pacific subpopulation that included A. purpurata was closest in genetic distance to a Southeast Asian subpopulation, suggesting frequent gene flow and supporting the hypothesis that the A. purpurata isolate arrived in Hawaii from Southeast Asia.

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