文献类型： 外文期刊

作者： Guo, Junjie ¹ ; Lian, Xijun ¹ ; Kang, Haiqi ² ; Gao, Kai ³ ; Li, Lin ⁴ ;

作者机构： 1.Tianjin Univ Commerce, Sch Biotechnol & Food Sci, Tianjin Key Lab Food Biotechnol, Tianjin 300134, Peoples R China

2.Sichuan Acad Agr Sci, Crop Res Inst, Chengdu 610066, Peoples R China

3.Natl Engn & Technol Res Ctr Preservat Agr Prod, Tianjin 300384, Peoples R China

4.South China Univ Technol, Coll Light Ind & Food Sci, Guangzhou 510640, Guangdong, Peoples R China

5.Guangdong Prov Key Lab Green Proc Nat Prod & Prod, Guangzhou 510640, Guangdong, Peoples R China

关键词： Anti-retrogradation;Glutenin;Amylose;Wheat starch;NMR

期刊名称：EUROPEAN FOOD RESEARCH AND TECHNOLOGY （ 影响因子：2.998； 五年影响因子：3.005 ）

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收录情况： SCI

摘要： Retrogradation is the process of starch recrystallization, and it profoundly affects the quality, acceptability and shelf-life of starch-containing foods. The influence of glutelin on the wheat starch retrogradation was studied in this paper. Glutenin was isolated from wheat flour, and its effect on retrogradation of wheat starch, amylose and amylopectin was investigated with UV-Vis (starch-iodine), IR and C-13 NMR. The results showed that glutenin probably interacted with amylose during gelation and retrogradation of starch. The results of IR showed that the addition of glutenin to wheat starch reduced the number of hydrogen bonds formed between amyloses during retrogradation. The C-13 NMR results suggested that tyrosine (Tyr) of glutenin might combine with amylose at the first carbon atom when they were mixed homogeneously and such combination was strengthened during retrogradation. Glutenin and amylose formed double helix with each other completely and hindered amylose-amylose short-term retrogradation of wheat starch, and glutenin would no longer inhibit the retrogradation of starch when all of the Tyr formed hydrogen bond with amylose. These results suggest that glutenin could play a powerful role in retarding the retrogradation of amylose, which is very important for the food industry.