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Screening and identification of a microsatellite marker associated with sex in Wami tilapia, Oreochromis urolepis hornorum

文献类型： 外文期刊

作者： Zhu, Huaping 1 ; Liu, Zhigang 1 ; Lu, Maixin 1 ; Gao, Fengying 1 ; Ke, Xiaoli 1 ; Ma, Dongmei 1 ; Huang, Zhanghan 2 ; Cao 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resource Applicat, Minist Agr, Guangzhou 510380, Guangdong, Peoples R China

2.Chinese Acad Fishery Sci, Pe

关键词： microsatellite;sex-linked marker;chromosome localization;fluorescence in situ hybridization;Oreochromis urolepis hornorum

期刊名称：JOURNAL OF GENETICS （ 影响因子：1.166； 五年影响因子：1.212 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In this study, primer pairs of 15 microsatellite markers associated with sex determination of tilapia were selected and amplified in Wami tilapia, Oreochromis urolepis hornorum. While one marker, UNH168, on linkage group 3 (LG3) was associated (P < 0.001) with the phenotypic sex in the experimental population, nine genotypes were detected in both sexes. Only 99-bp allele was detected in the female samples, while 141, 149 and 157-bp alleles were present in both male and female samples. UNH168 was localized by fluorescence in situ hybridization (FISH) on the long arm of the largest tilapia chromosome pair (chromosome 1, equivalent to LG3). This sex-linked microsatellite marker could potentially be used for marker-assisted selection in tilapia breeding programmes to produce monosex male tilapia.

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