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The binding, synergistic and structural characteristics of BsEXLX1 for loosening the main components of lignocellulose: Lignin, xylan, and cellulose

文献类型： 外文期刊

作者： Wang, Qun 1 ; Chen, Liang 2 ; Lin, Hui 3 ; Yu, Daobing 4 ; Shen, Qi 1 ; Wan, Li 5 ; Zhao, Yuhua 1 ;

作者机构： 1.Zhejiang Univ, Inst Microbiol, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China

2.Zhejiang Gongshang Univ, Hangzhou 310018, Zhejiang, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Environm Resource & Soil Fertilizer, Hangzhou 310021, Zhejiang, Peoples R China

4.Zhejiang Agr & Forestry Univ, Sch Forestry & Biotechnol, Lin An 311300, Zhejiang, Peoples R China

5.Vanderbilt Univ, Dept Chem & Biomol Engn, Nashville, TN 37235 USA

关键词： Lignocellulose loosening;BsEXLX1;Site-directed mutagenesis;Synergistic activity;Homologous modeling

期刊名称：ENZYME AND MICROBIAL TECHNOLOGY （ 影响因子：3.493； 五年影响因子：3.699 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The bacterial expansin, BsEXLX1, has been studied as a model to understand the synergistic effect of expansins on lignocellulose degradation and the structure-function relationships of expansins. However, the specific mechanism is still poorly understood. In this study, the binding, synergistic and structural characteristics of BsEXDU for loosening the main components of lignocellulose: lignin, xylan, and cellulose, were further characterized. Results showed that BsEXLX1 preferentially binds to xylan over lignin or cellulose under various conditions. The binding of BsEXLX1 to all substrates increased linearly with the initial concentration of BsEXLX1. But the changing rate of binding (i.e., slope of the line; k value) varied with the incubation temperature. Interestingly, the binding of BsEXLX1 to substrates did not saturate. Mutating residue-125, 126 or 171 indicated their importance for binding, but they were less important for binding to xylan. Through binding assays and homologous modeling, it was concluded that residue 125 and 171 play more important roles in the structural maintenance of BsEXLX1. By comparing the synergistic activity of BsEXLX1 or its mutants, it was found that synergistic activity is not correlated with specific binding. All these results can lead deeper understand about the mechanism of wall loosening by expansins, and further promote the application of expansins in lignocellulose degradation. (C) 2016 Elsevier Inc. All rights reserved.

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