文献类型： 外文期刊

作者： Li, Lan-Song ¹ ; Meng, Yu-Ping ² ; Cao, Qiu-Fen ² ; Yang, Yong-Zhen ³ ; Wang, Fan ² ; Jia, Hu-Sheng ³ ; Wu, Shu-Biao ² ;

作者机构： 1.Taiyuan Univ Technol, Coll Chem & Chem Engn, Taiyuan 030024, Peoples R China

2.Shanxi Acad Agr Sci, Biotechnol Res Ctr, Taiyuan 030031, Peoples R China

3.Taiyuan Univ Technol, Minist Educ, Key Lab Interface Sci & Engn Adv Mat, Taiyuan 030024, Peoples R China

4.Univ New England, Sch Environm & Rural Sci, Armidale, NSW 2351, Australia

关键词： Z. jujuba Mill.;metallothionein;heavy metal tolerance;heavy metal hyperaccumulation

期刊名称：BIOCHEMISTRY-MOSCOW （ 影响因子：2.487； 五年影响因子：2.57 ）

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收录情况： SCI

摘要： Metallothioneins (MTs) are a family of low molecular weight, cysteine-rich, metal-binding proteins that are able to make cells to uptake heavy metals from the environment. Molecular and functional characterization of this gene family improves understanding of the mechanisms underlying heavy metal tolerance in higher organisms. In this study, a cDNA clone, encoding 74-a.a. metallothionein type 1 protein (ZjMT), was isolated from the cDNA library of Ziziphus jujuba. At the N- and C-terminals of the deduced amino acid sequence of ZjMT, six cysteine residues were arranged in a CXCXXXCXCXXXCXC and CXCXXXCXCXXCXC structure, respectively, indicating that ZjMT is a type 1 MT. Quantitative PCR analysis of plants subjected to cadmium stress showed enhanced expression of ZjMT gene in Z. jujuba within 24 h upon Cd exposure. Escherichia coli cells expressing ZjMT exhibited enhanced metal tolerance and higher accumulation of metal ions compared with control cells. The results indicate that ZjMT contributes to the detoxification of metal ions and provides marked tolerance against metal stresses. Therefore, ZjMT may be a potential candidate for tolerance enhancement in vulnerable plants to heavy metal stress and E. coli cells containing the ZjMT gene may be applied to adsorb heavy metals in polluted wastewater.