您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Swarming motility plays the major role in migration during tomato root colonization by Bacillus subtilis SWR01

文献类型： 外文期刊

作者： Gao, Shengfeng 1 ; Wu, Huijun 1 ; Yu, Xinfang 1 ; Qian, Limin 1 ; Gao, Xuewen 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Key Lab Integrated Management Crop Dis & Pests, Minist Educ, Nanjing, Jiangsu, Peoples R China

2.Chinese Acad Trop Agr Sci, Spice & Beverage Res Inst, Haikou, Hainan Province, Peoples R China

关键词： Root colonization;Bacillus subtilis SWR01;Swarming;Chemotaxis;Flagella motility

期刊名称：BIOLOGICAL CONTROL （ 影响因子：3.687； 五年影响因子：3.962 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Root colonization is important for the application of Plant Growth-Promoting Rhizobacteria (PGPR). Previously, chemotaxis was believed to be the major trait for colonization. However, because chemotaxis defective mutants are usually swarming impaired at the same time, in this study we clarified the roles played by swarming motility and chemotaxis in tomato root colonization by Bacillus subtilis SWR01. Tomato seeds were treated with B. subtilis SWR01 or mutants before being sown in Simons' gnotobiotic system, and the colonization efficacy at root tips were observed after 2 weeks. Both microscopy and plate counts showed that the colonization efficacy of swarming defective and chemotaxis proficient mutants (Delta swrA, Delta minJ and Delta srfAC) was about 5%-15% that of the parental strain SWR01, while the colonization efficacy of the swarming normal and chemotaxis impaired mutant Delta cheV was 78.9% that of the wild-type bacteria. These results demonstrate that while both chemotaxis and swarming motility are important in root colonization, and the role played by swarming is greater than that of chemotaxis. In addition, non-flagellated mutants (Delta hag) showed 0.47% colonization efficacy relative to that of B. subtilis SWR01, suggesting more than 99% root colonization requires the presence of flagella. This study indicates new ways to enhance PGPR rhizoplane colonization, which is useful in agricultural applications. (C) 2016 Elsevier Inc. All rights reserved.

  • 相关文献

[1]Diversity and chemotaxis of soil bacteria with antifungal activity against Fusarium wilt of banana. Li, Ping,Ma, Li,Feng, Yun Li,Mo, Ming He,Li, Ping,Ma, Li,Feng, Yun Li,Mo, Ming He,Yang, Fa Xiang,Dai, Hao Fu,Zhao, You Xing. 2012

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台