Swarming motility plays the major role in migration during tomato root colonization by Bacillus subtilis SWR01
文献类型: 外文期刊
作者: Gao, Shengfeng 1 ; Wu, Huijun 1 ; Yu, Xinfang 1 ; Qian, Limin 1 ; Gao, Xuewen 1 ;
作者机构: 1.Nanjing Agr Univ, Coll Plant Protect, Key Lab Integrated Management Crop Dis & Pests, Minist Educ, Nanjing, Jiangsu, Peoples R China
2.Chinese Acad Trop Agr Sci, Spice & Beverage Res Inst, Haikou, Hainan Province, Peoples R China
关键词: Root colonization;Bacillus subtilis SWR01;Swarming;Chemotaxis;Flagella motility
期刊名称:BIOLOGICAL CONTROL ( 影响因子:3.687; 五年影响因子:3.962 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Root colonization is important for the application of Plant Growth-Promoting Rhizobacteria (PGPR). Previously, chemotaxis was believed to be the major trait for colonization. However, because chemotaxis defective mutants are usually swarming impaired at the same time, in this study we clarified the roles played by swarming motility and chemotaxis in tomato root colonization by Bacillus subtilis SWR01. Tomato seeds were treated with B. subtilis SWR01 or mutants before being sown in Simons' gnotobiotic system, and the colonization efficacy at root tips were observed after 2 weeks. Both microscopy and plate counts showed that the colonization efficacy of swarming defective and chemotaxis proficient mutants (Delta swrA, Delta minJ and Delta srfAC) was about 5%-15% that of the parental strain SWR01, while the colonization efficacy of the swarming normal and chemotaxis impaired mutant Delta cheV was 78.9% that of the wild-type bacteria. These results demonstrate that while both chemotaxis and swarming motility are important in root colonization, and the role played by swarming is greater than that of chemotaxis. In addition, non-flagellated mutants (Delta hag) showed 0.47% colonization efficacy relative to that of B. subtilis SWR01, suggesting more than 99% root colonization requires the presence of flagella. This study indicates new ways to enhance PGPR rhizoplane colonization, which is useful in agricultural applications. (C) 2016 Elsevier Inc. All rights reserved.
- 相关文献
作者其他论文 更多>>
-
Fusaricidin produced by the rhizobacterium Paenibacillus polymyxa NX20 is involved in the biocontrol of postharvest plant-pathogenic oomycete Phytophthora capsici
作者:Yu, Chenjie;Yang, Xihao;Liang, Xiaoli;Song, Yan;Zhu, Linli;Xing, Shuyue;Gu, Qin;Dong, Suomeng;Gao, Xuewen;Wu, Huijun;Yang, Yang;Borriss, Rainer
关键词:Paenibacillus polymyxa; Phytophthora capsici; Postharvest; Fusaricidin; Inhibitory activity; Anti-oomycete mechanisms
-
Identification and screening of fungicides against Piper nigrum basal Fusarium wilt disease in Hainan, China
作者:Liu, Shichao;Liu, Ruibing;Meng, Qianqian;Gou, Yafeng;Xue, Chao;Tian, Tian;Wen, Siwei;Sun, Shiwei;Gao, Shengfeng;Liu, Shichao;Liu, Ruibing;Meng, Qianqian;Gou, Yafeng;Xue, Chao;Tian, Tian;Wen, Siwei;Sun, Shiwei;Gao, Shengfeng;Chu, Bo;Li, Zhuang;Chen, Pengyun;Wei, Fei;Liu, Yanan;Sun, Shiwei;Gao, Shengfeng
关键词:fungicide; Fusarium solani; Fusarium wilt; pathogen identification; Piper nigrum
-
The Virome of Piper nigrum: Identification, Genomic Characterization, Prevalence, and Transmission of Three New Viruses of Black Pepper in China
作者:Ma, Yuxin;Che, Haiyan;Lin, Yating;Li, Shifang;Ma, Yuxin;Xing, Fei;Li, Shifang;Gao, Shengfeng
关键词:badnavirus; enamovirus; fabavirus; high-throughput sequencing; novel viruses; Piper nigrum; transmission; viral disease
-
Diverse Novel Viruses Coinfecting the Tropical Ornamental Plant Polyscias balfouriana in China
作者:Ma, Yuxin;Che, Haiyan;Lin, Yating;Li, Shifang;Ma, Yuxin;Gao, Shengfeng;Li, Shifang
关键词:Polyscias balfouriana; virus discovery; coinfection; ornamental plant; Betaflexiviridae; Closteroviridae; Capillovirus; Citrivirus; Crinivirus
-
Selection of reference genes for quantitative real-time PCR normalization in the coffee white stem borer, Xylotrechus quadripes Chevrolat (Coleoptera: Cerambycidae)
作者:Meng, Qianqian;Sun, Shiwei;Wang, Ying;Yang, Mei;Zhu, Enhang;Liu, Aiqin;Gao, Shengfeng;Gou, Yafeng;Wang, Zheng;Shu, Benshui;Wang, Ying;Yang, Mei;Zhu, Enhang
关键词:Abiotic factors; biotic factors; expression stability; qRT-PCR; reference genes; Xylotrechus quadripes Chevrolat
-
Diverse Novel Viruses Coinfecting the Tropical Ornamental Plant Polyscias balfouriana in China
作者:Ma, Yuxin;Che, Haiyan;Lin, Yating;Li, Shifang;Ma, Yuxin;Gao, Shengfeng;Li, Shifang
关键词:Polyscias balfouriana; virus discovery; coinfection; ornamental plant; Betaflexiviridae; Closteroviridae; Capillovirus; Citrivirus; Crinivirus
-
The Virome of Piper nigrum: Identification, Genomic Characterization, Prevalence, and Transmission of Three New Viruses of Black Pepper in China
作者:Ma, Yuxin;Che, Haiyan;Lin, Yating;Li, Shifang;Ma, Yuxin;Xing, Fei;Li, Shifang;Gao, Shengfeng
关键词:badnavirus; enamovirus; fabavirus; high-throughput sequencing; novel viruses; Piper nigrum; transmission; viral disease