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Ectopic Expression of BnaC.CP20.1 Results in Premature Tapetal Programmed Cell Death in Arabidopsis

文献类型： 外文期刊

作者： Song, Liping 1 ; Zhou, Zhengfu; Tang, Shan 1 ; Zhang, Zhiqiang 1 ; Xia, Shengqian 1 ; Qin, Maomao 1 ; Li, Bao 1 ; Wen, 1 ;

作者机构： 1.Huazhong Agr Univ, Natl Ctr Rapeseed Improvement Wuhan, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China

2.Huazhong Agr Univ, Natl Ctr Rape

关键词： BnaC.CP20.1;Brassica napus;Cysteine proteases;Male sterility;Pollen exine;Tapetal PCD

期刊名称：PLANT AND CELL PHYSIOLOGY （ 影响因子：4.927； 五年影响因子：5.516 ）

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收录情况： SCI

摘要： Tapetal programmed cell death (PCD) is essential in pollen grain development, and cysteine proteases are ubiquitous enzymes participating in plant PCD. Although the major papain-like cysteine proteases (PLCPs) have been investigated, the exact functions of many PLCPs are still poorly understood in PCD. Here, we identified a PLCP gene, BnaC.CP20.1, which was closely related to XP_013596648.1 from Brassica oleracea. Quantitative real-time PCR analysis revealed that BnaC.CP20.1 expression was down-regulated in male-sterile lines in oilseed rape, suggesting a connection between this gene and male sterility. BnaC.CP20.1 is especially active in the tapetum and microspores in Brassica napus from the uninucleate stage until formation of mature pollen grains during anther development. On expression of BnaC.CP20.1 prior to the tetrad stage, BnA9::BnaC.CP20.1 transgenic lines in Arabidopsis thaliana showed a male-sterile phenotype with shortened siliques containing fewer or no seeds by self-crossing. Scanning electron microscopy indicated that the reticulate exine was defective in aborted microspores. Callose degradation was delayed and microspores were not released from the tetrad in a timely fashion. Additionally, the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay indicated that BnaC.CP20.1 ectopic expression led to premature tapetal PCD. Transmission electron microscopy analyses further demonstrated that the pollen abortion was due to the absence of tectum connections to the bacula in the transgenic anthers. These findings suggest that timely expression of BnaC.CP20.1 is necessary for tapetal degeneration and pollen wall formation.

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[1]Cysteine Protease 51 (CP51), an anther-specific cysteine protease gene, is essential for pollen exine formation in Arabidopsis. Yang, Yongxue,Dong, Caihua,Yu, Jingyin,Tong, Chaobo,Huang, Junyan,Liu, Shengyi,Yang, Yongxue,Shi, Lei,Li, Zhenbo.

[2]SCAR and RAPD markers associated with 18-carbon fatty acids in rapeseed, Brassica napus. Hu, J,Li, G,Struss, D,Quiros, CF. 1999

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