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Self-paired monoclonal antibody lateral flow immunoassay strip for rapid detection of Acidovorax avenae subsp citrulli

文献类型： 外文期刊

作者： Zeng, Haijuan 1 ; Guo, Wenbo 2 ; Liang, Beibei 3 ; Li, Jianwu 1 ; Zhai, Xuzhao 1 ; Song, Chunmei 1 ; Zhao, Wenjun 4 ; Fan 1 ;

作者机构： 1.Univ Shanghai Sci & Technol, Sch Med Instrument & Food Engn, Shanghai 200093, Peoples R China

2.Shanghai Acad Agr Sci, Inst Agri Food Stand & Testing Technol, Shanghai 201403, Peoples R China

3.Shanghai Univ Med & Hlth Sci, Shanghai 200093, Peoples R China

4.Chinese Acad Inspect & Quarantine, Beijing 100029, Peoples R China

5.Univ Freiburg, Inst Biochem & Mol Biol, ZBMZ, D-79104 Freiburg, Germany

关键词： Acidovorax avenae subsp;citrulli;Monoclonal antibody;Self-paired;Colloidal gold;Immunoassay;Test strip

期刊名称：ANALYTICAL AND BIOANALYTICAL CHEMISTRY （ 影响因子：4.142； 五年影响因子：3.863 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： We screened a highly specific monoclonal antibody (McAb), named 6D, against Acidovorax avenae subsp. citrulli (Aac). Single McAb 6D was used as both nanogold-labeled antibody and test antibody to develop a single self-paired colloidal gold immunochromatographic test strip (Sa-GICS). The detection limit achieved using the Sa-GICS approach was 10(5) CFU/mL, with a result that can be observed by the naked eye within 10 min. Moreover, Sa-GICS can detect eight strains of Aac and display no cross-reactions with other pathogenic plant microorganisms. Artificial contamination experiments demonstrated that Sa-GICS would not be affected by impurities in the leaves or stems of the plants and were consistent with the PCR results. This is the first report on the development of a colloidal gold immunoassay strip with self-paired single McAb for the rapid detection of Aac.

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