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A characterization of grapevine of GRAS domain transcription factor gene family

文献类型： 外文期刊

作者： Sun, Xin 1 ; Xie, Zhengqiang 1 ; Zhang, Cheng 1 ; Mu, Qian 1 ; Wu, Weimin 3 ; Wang, Baoju 1 ; Fang, Jinggui 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China

2.Jiangsu Polytech Coll Agr & Forestry, Dept Agron & Hort, Jurong 212400, Jiangsu, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Hort, Nanjing 210014, Jiangsu, Peoples R China

关键词： Grapevine;GRAS gene family;Expression;Function

期刊名称：FUNCTIONAL & INTEGRATIVE GENOMICS （ 影响因子：3.41； 五年影响因子：3.616 ）

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收录情况： SCI

摘要： GRAS domain genes are a group of important plant-specific transcription factors that have been reported to be involved in plant development. In order to know the roles of GRAS genes in grapevine, a widely cultivated fruit crop, the study on grapevine GRAS (VvGRAS) was carried out, and from which, 43 were identified from 12x assemble grapevine genomic sequences. Further, the genomic structures, synteny, phylogeny, expression profiles in different tissues of these genes, and their roles in response to stress were investigated. Among the genes, two potential target genes (VvSCL15 and VvSCL22) for VvmiR171 were experimentally verified by PPM-RACE and RLM-RACE, in that not only the cleavage sites of miR171 on the target mRNA were mapped but also the cleaved fragments and their expressing patterns were detected. Transgenic Arabidopsis plants over expression VvSCL15 showed lower tolerance to drought and salt treatments.

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