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Production of germline transgenic pigs co-expressing double fluorescent proteins by lentiviral vector

文献类型： 外文期刊

作者： Chen, Xiao-yu 1 ; Zhu, Zhi-wei 1 ; Yu, Fu-xian 1 ; Huang, Jing 1 ; Hu, Xiao-rui 1 ; Pan, Jian-zhi 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Sci, Hangzhou, Zhejiang, Peoples R China

关键词： Pig;Germline;Lentivirus;Double-transgenic;Perivitelline injection

期刊名称：ANIMAL REPRODUCTION SCIENCE （ 影响因子：2.145； 五年影响因子：2.281 ）

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收录情况： SCI

摘要： Genomic integration of transgene by lentiviral vector has been proved an efficient method to produce single-transgenic animals. But it failed to create multi-gene transgenic offspring. Here, we have exploited lentivirus to generate the double-transgenic piglets through the female germline. The recombinant lentivirus containing fluorescent proteins genes (DsRed1 and Venus) were injected into the perivitelline space of 2-cell stage in vitro porcine embryos.

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