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Efficient regeneration and transformation of Spathiphyllum cannifolium

文献类型： 外文期刊

作者： Yu, Bo 1 ; Liao, Feixiong 1 ; Liu, Jinmei 1 ; Sun, Yingbo 1 ; Huang, Lili 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Environm Hort Inst, Guangzhou 510640, Guangdong, Peoples R China

关键词： Spathiphyllum cannifolium;Embryogenic suspension cultivation;Plant regeneration;Agrobacterium tumefaciens;Transformation

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）

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收录情况： SCI

摘要： An efficient and stable transformation system was developed based on embryogenic cell suspension culture by Agrobacterium tumefaciens-mediated method in Spathiphyllum cannifolium. Embryogenic calli induced from leaves on MS medium with 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mg/L thidiazuron were incubated in liquid medium with identical growth regulators for suspension cultivation. The plantlet regeneration rate of embryogenic suspension cell aggregates (ESCAs) incubated on MS medium achieved 100 %. The ESCAs were transformed using A. tumefaciens strain EHA105 harboring a vector pCAMBIA1301 containing the beta-glucuronidase (gusA) reporter gene and the hygromycin phosphotransferase II (hpt II) selection marker gene. The optimized transformation conditions were as follows: infiltration of ESCAs with Agrobacterium for 10 min under 60 kPa vacuum pressure, followed by co-cultivation on medium containing 200 mu M acetosyringone for 3 days. In total, 858 plants were regenerated from 1200 infected ESCAs. Analyses of randomly selected regenerated plants by GUS assays, PCR and dot blot showed that 91.30 % were transgenic. Southern blot analyses revealed that the copy number of T-DNAs integrated into the genome ranged from one to three. This newly developed transformation system provides a basis for the future development of genetic engineering techniques to improve flower color and enhance the disease resistance of peace lily.

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