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Application of DNA aptamers as sensing layers for detection of carbofuran by electrogenerated chemiluminescence energy transfer

文献类型： 外文期刊

作者： Li, Shuhuai 1 ; Wu, Xuejin 1 ; Liu, Chunhua 1 ; Yin, Guihao 1 ; Luo, Jinhui 1 ; Xu, Zhi 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Anal & Test Ctr, Haikou 571101, Peoples R China

2.Minist Agr, Lab Qual & Safety Risk Assessment Trop Prod Haiko, Haikou 571101, Peoples R China

关键词： Aptasensor;Fullerene;Electrogenerated chemiluminescence;Gold nanoparticles;Carbofuran

期刊名称：ANALYTICA CHIMICA ACTA （ 影响因子：6.558； 五年影响因子：6.228 ）

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收录情况： SCI

摘要： In this study, an electrogenerated chemiluminescence (ECL) sensing platform for carbofuran detection was constructed based on ECL energy transfer (ECRET) and carbon dot (C-dot)-tagged aptamers as the recognition element. Fullerene (C60)-loaded gold nanoparticles (C60-Au) were used as the energy donor, modified on a glassy carbon electrode. C-dot -tagged DNA aptamers were used as the receptor, and ECRET then occurred between C60-Au and C-dots. After accepting the energy, the C-dots acted as a signal indicator and showed decreased signal intensity in the presence of targets, which competitively bound to DNA aptamers and blocked energy transfer. Using this robust, straight-forward strategy, the sensor showed a linear ECL response to carbofuran at concentrations from 2.0 x 10(-11) mol L-1 to 8.0 x 10(-9) mol L-1. The detection limit of this assay was shown to be 8.8 x 10(-13) mol L-1. Thus, the sensing approach described in this study could be adapted for use in the detection of various pesticide residue targets. (C) 2016 Elsevier B.V. All rights reserved.

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[1]Supramolecular imprinted sensor for carbofuran detection based on a functionalized multiwalled carbon nanotube-supported Pd-Ir composite and methylene blue as catalyst. Li, Shuhuai,Yin, Guihao,Wu, Xuejin,Liu, Chunhua,Luo, Jinhui,Li, Shuhuai,Yin, Guihao,Wu, Xuejin,Liu, Chunhua,Luo, Jinhui.

[2]Synergetic dual recognition and separation of the fungicide carbendazim by using magnetic nanoparticles carrying a molecularly imprinted polymer and immobilized beta-cyclodextrin. Li, Shuhuai,Wu, Xuejin,Zhang, Qun,Li, Pingping,Li, Shuhuai,Wu, Xuejin,Zhang, Qun,Li, Pingping.

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