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Proteolytic Activation of Bacillus thuringiensis Cry2Ab through a Belt-and-Braces Approach

文献类型： 外文期刊

作者： Xu, Lian 1 ; Pan, Zhi-Zhen 2 ; Zhang, Jing 1 ; Liu, Bo 2 ; Zhu, Yu-Jing 2 ; Chen, Qing-Xi 1 ;

作者机构： 1.Xiamen Univ, Sch Life Sci, State Key Lab Cellular Stress Biol, Key Lab,Minist Educ Coastal & Wetland Ecosyst, Xiamen 361005, Fujian, Peoples R China

2.Fujian Acad Agr Sci, Agr Bioresources Res Inst, Fuzhou 350003, Fujian, Peoples R China

关键词： Bacillus thuringiensis;Cry2Ab;proteolysis;cleavage site;insecticidal activity

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

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收录情况： SCI

摘要： Proteolytic processing of Bacillus thuringiensis (Bt) crystal toxins by insect midgut proteases plays an essential role in their insecticidal toxicities against target insects. In the present study, proteolysis of Bt crystal toxin Cry2Ab by Plutella xylostella L. midgut proteases (PxMJ) was, evaluated. Both trypsin and chymotrypsin were identified involving the proteolytic activation of Cry2Ab and cleaving Cry2Ab at Arg(139) and Leu(144), respectively. Three Cry2Ab mutants (R139A, L144A, and R139A-L144A) were constructed by replacing residues Arg(139), Leu(144), and Arg(139)-Leu(144) with alanine. Proteolysis assays revealed that mutants R139A and L144A but not R139A-L144A could be cleaved into SO kDa activated toxins by PxMJ. Bioassays showed that mutants R139A and L144A were highly toxic against P. xylostella larvae, while mutant R139A-L144A was almost non insecticidal. Those results demonstrated that proteolysis by PxMJ was associated with the toxicity of Cry2Ab against P. xylostella. It also revealed that either trypsin or chymotrypsin was enough to activate Cry2Ab protoxin.;This characteristic was regarded as a belt-and-braces approach and might contribute to the control of resistance development in target insects. Our studies characterized the proteolytic processing of Cry2Ab and provided new insight into the activation of this Bt toxin.

  • 相关文献

[1]Proteolytic activation of Bacillus thuringiensis Vip3Aa protein by Spodoptera exigua midgut protease. Zhang, Ling,Xu, Lian,Li, Jie,Niu, Li -Yang,Chen, Qing-Xi,Pan, Zhi-Zhen,Liu, Bo,Chen, Zheng,Zhu, Yu-Jing. 2018

[2]Characterization of a new cry2Ab gene of Bacillus thuringiensis with high insecticidal activity against Plutella xylostella L.. Pan, Zhizhen,Zhu, Yujing,Shi, Huai,Chen, Zheng,Chen, Meichun,Liu, Bo,Xu, Lian,Chen, Qingxi.

[3]PxAPN5 serves as a functional receptor of Cry2Ab in Plutella xylostella (L.) and its binding domain analysis. Pan, Zhi-Zhen,Liu, Bo,Chen, Zheng,Zhu, Yu-Jing,Xu, Lian,Zhang, Jing,Chen, Qing-Xi.

[4]Insecticidal activity and antifeedant effect of a new type biocide GCSC-BtA against Plutella xylostella L. (Lep., Plutellidae). Sengonca, C,Liu, B,Zhu, YJ. 2006

[5]小菜蛾钙黏蛋白PxCR_(10-11)结构域的克隆、原核表达及功能分析. 许炼,刘波,潘志针,朱育菁,高焕娟,陈清西. 2017

[6]Screening of Vip3Aa60 and Vip3Ad5 and characterization of their binding to Spodoptera exigua midguts. Pan, Zhi-Zhen,Guan, Xiong,Xu, Lian,Zhang, Jing,Chen, Qing-Xi,Gao, Huan-Juan,Pan, Zhi-Zhen,Liu, Bo,Chen, Zheng,Zhu, Yu-Jing. 2017

[7]Effect of chemical additives on Bacillus thuringiensis (Bacillales: Bacillaceae) against Plutella xylostella (Lepidoptera: Pyralidae). Zhang, L.,Huang, T.,Huang, Z.,Xu, L.,Wu, C.,Guan, X.,Qiu, S.,Gelbic, I..

[8]Effect of GCSC-BtA biocide on abundance and diversity of some cabbage pests as well as their natural enemies in southeastern China. Sengonca, C,Liu, B.

[9]Laboratory studies on the effect of the bioinsecticide GCSC-BtA on mortality and feeding of diamondback moth Plutella xylostella L. (Lepidoptera : Plutellidae) larvae on cabbage. Liu, B.,Zhu, Y. J.,Sengonca, C.. 2006

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