文献类型： 外文期刊
作者： Chen, Hui-Ling 1 ; Guo, Meng-Meng 2 ; Tang, Hao 1 ; Wu, Zhan 1 ; Tang, Li-Juan 1 ; Yu, Ru-Qin 1 ; Jiang, Jian-Hui 1 ;
作者机构： 1.Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
期刊名称：ANALYTICAL METHODS （ 影响因子：2.896； 五年影响因子：2.716 ）
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收录情况： SCI
摘要： MicroRNAs (miRNAs) are quite short single-stranded RNA molecules playing crucial roles in many biological processes and recognized as potential diagnostic biomarkers as well as targets for drug discovery in cancers. It has fueled a great need for the development of highly sensitive and selective detection methods for miRNAs. Many nucleic acid amplification technologies are demonstrated methods which have exhibited great potential for the development of simple, sensitive, specific and high-throughput methods for the detection of miRNA. Herein, we review the basic principles of five types of nucleic acid amplification-based miRNA assay methods that have been established in recent three years. Sensitive miRNA detection based on polymerase chain reaction, rolling circle amplification, strand displacement amplification, duplex-specific nuclease signal amplification, and hybridization chain reaction techniques is discussed.
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