Identification and Localization of beta-D-Glucosidase from Two Typical Oenococcus oeni Strains
文献类型： 外文期刊
作者： Li, Yahui 1 ; Ma, Yanhong 1 ; Huang, Kaihong 1 ; Zhang, Hongzh 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Agro Prod Proc, Nanjing, Jiangsu, Peoples R China
关键词： Oenococcus oeni;beta-D-glucosidase;beta G cloned;gene localization
期刊名称：POLISH JOURNAL OF MICROBIOLOGY （ 影响因子：1.28； 五年影响因子：1.352 ）
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收录情况： SCI
摘要： beta-D-glucosidase (beta G) gene from Oenococcus oeni SD-2a and 31MBR was cloned, sequenced and analyzed, also intracellular beta G of the two strains was further localized. The results showed that beta G gene of the two strains was in high homology (>99%) to reported 13G gene, confirming both strains possess beta G activity at the molecular level. Intracellular 13G of SD-2a is a mainly soluble protein, existing mostly in the cytoplasm and to some extent in the periplasm. While for 31MBR, intracellular beta G is mainly insoluble protein existing in the cytoplasmic membrane. This study provides basic information for further study of the metabolic mechanism of beta G from O. oeni SD-2a and 3IMBR.
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