Identification and Localization of beta-D-Glucosidase from Two Typical Oenococcus oeni Strains
文献类型： 外文期刊
作者： Li, Yahui 1 ; Ma, Yanhong 1 ; Huang, Kaihong 1 ; Zhang, Hongzh 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Agro Prod Proc, Nanjing, Jiangsu, Peoples R China
关键词： Oenococcus oeni;beta-D-glucosidase;beta G cloned;gene localization
期刊名称：POLISH JOURNAL OF MICROBIOLOGY （ 影响因子：1.28； 五年影响因子：1.352 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： beta-D-glucosidase (beta G) gene from Oenococcus oeni SD-2a and 31MBR was cloned, sequenced and analyzed, also intracellular beta G of the two strains was further localized. The results showed that beta G gene of the two strains was in high homology (>99%) to reported 13G gene, confirming both strains possess beta G activity at the molecular level. Intracellular 13G of SD-2a is a mainly soluble protein, existing mostly in the cytoplasm and to some extent in the periplasm. While for 31MBR, intracellular beta G is mainly insoluble protein existing in the cytoplasmic membrane. This study provides basic information for further study of the metabolic mechanism of beta G from O. oeni SD-2a and 3IMBR.
- 相关文献
作者其他论文 更多>>
-
Multiscale pore drives mechanical properties and storage stability in peach crisps
作者：Zhao, Han;Zhang, Bo;Xiang, Kexin;Li, Dajing;Liu, Chunju;Niu, Liying;Ma, Yanhong;Xiao, Yadong;Zhao, Han;Zhang, Bo;Xiang, Kexin;Liu, Chunju;Wang, Haiou;Liu, Chunju
关键词：Peach; Micro/nanopore structure; Pore distribution; Mechanical properties; Storage stability
-
Analysis of the effect of three different blanching processes on the flavor profile of peeled and unpeeled broad beans
作者：Shi, Lu;Fu, Xiaohui;Lin, Menghua;Li, Yahui;Liang, Ying;Zhang, Zhiyong;Fu, Xiaohui
关键词：Broad bean; Blanching; HS-GC-IMS; Volatile compounds; Antioxidant activity
-
Optimization of Ultrasonic-Enzymatic-Assisted Extraction of Flavonoids from Sea Buckthorn (Hippophae rhamnoides L.) Pomace: Chemical Composition and Biological Activities
作者：Suo, Wenyu;Suo, Wenyu;Wang, Wenzhe;Li, Dajing;Wu, Haihong;Huang, Wuyang;Ma, Yanhong;Liu, Haiyan
关键词：
Hippophae rhamnoides L.; flavonoids; ultrasonic-enzymatic-assisted extraction; antioxidant; alpha-glucosidase and alpha-amylase inhibition -
TOLLIP inhibits the replication of PEDV by autophagic degradation of Nsp9
作者：Li, Yahui;Yang, Xianghong;Li, Bin;Li, Yahui;Zhang, Yutao;Cheng, Jiexi;Hu, Boli;Li, Yahui;Chen, Jinyang;Lin, Zhiwei
关键词：Autophagy cargo receptors; PEDV; TOLLIP; Selective autophagy; LC3A
-
Screening of hypoglycemic and probiotic properties of Pediococcus acidilactici FM-Pa-JXM94 and exploring the potential hypoglycemic mechanism
作者：Wang, Ying;Zhang, Hui;Wang, Xingna;Zhang, Yu;Fan, Linlin;Ma, Yanhong;Liu, Xiaoli;Wang, Ying;Zhang, Hui;Liu, Xiaoli;Wang, Ying
关键词：Pediococcus acidilactici; alpha-Amylase and alpha-glucosidase inhibition; activities; Lactic acid bacteria; Probiotic properties; Hypoglycemic function
-
Comprehensive analysis of volatile flavor components in pear fruit spanning the entire development stages
作者：Shi, Lu;He, Wanlin;Lin, Menghua;Fu, Xiaohui;Li, Yahui;Liang, Ying;Zhang, Zhiyong;He, Wanlin;Liang, Ying;Fu, Xiaohui
关键词：Volatile compounds; Organic acids; Fatty acid; Ripening process; Esters biosynthetic genes; Pear
-
Investigation of the formation of furfural compounds in apple products treated with pasteurization and high pressure processing
作者：Gao, Qingchao;Wang, Yingxin;Li, Yahui;Liang, Ying;Zhang, Zhiyong;Wang, Yingxin;Liang, Ying;Hou, Jianbo
关键词：Furfurals; Apple; HPLC-DAD; Pasteurization; High pressure processing
