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Molecular cloning, characterization and evolutionary analysis of vitellogenin in Chinese giant salamander Andrias davidianus

文献类型： 外文期刊

作者： Tian, Hai-feng 1 ; Meng, Yan 1 ; Hu, Qiao-mu 1 ; Xiao, Han-bing 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China

2.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Jiangsu, Peoples R China

关键词： vitellogenin;Andrias davidianus;tissue expression;phylogenetic analysis

期刊名称：BIOLOGIA （ 影响因子：1.35； 五年影响因子：1.139 ）

ISSN：

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收录情况： SCI

摘要： Vitellogenin (VTG) is the major precursor of yolk proteins and could transport many components into oocytes, and hence it was thought to be a potential marker of reproduction of oviparious animal. Andrias davidianus is a critically endangered amphibian species, of which the wild population decreased dramatically and the artificial breeding has been successfully established. In this study, a full-length VTG cDNA was cloned and characterized from A. davidianus (designated as AdVTG) using 5' and 3' rapid amplification cDNA ends and RT-PCR. The AdVTG cDNA is 5,418 base pair in length and it encodes 1,805 amino acid long protein with typical quadripartite domains and several conserved cleavage sites. The tissue expression pattern indicated that AdVTG is mainly expressed in liver in one-year old individual. The three-dimensional model of AdVTG constructed by homology modelling revealed the presence of conserved N-terminal beta-sheet and lipid-binding cavity. Moreover, the phylogenetic analysis of VTG in metazoa was carried out, which revealed that Anura-specific gene duplication occurred after the divergence of Caudata. All these results would improve the understanding of the physiological roles of AdVTG in A. davidianus and contribute to elucidating the evolution of VTG in vertebrates.

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