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Gga-miR-21 inhibits chicken pre-adipocyte proliferation in part by down-regulating Kruppel-like factor 5

文献类型： 外文期刊

作者： Wang, Weishi 1 ; Cheng, Min 1 ; Qiao, Shupei 1 ; Wang, Yuxiang 1 ; Li, Hui 1 ; Wang, Ning 1 ;

作者机构： 1.Minist Agr, Key Lab Chicken Genet & Breeding, Harbin, Peoples R China; Educ Dept Heilongjiang Prov, Key Lab Anim Genet Breeding & Reprod, Harbin, Peoples R China; Northeast Agr Univ, Coll Anim Sci & Technol, Harbin, Peoples R China

关键词： ga-miR-21;KLF5;pre-adipocyte;proliferation

期刊名称：POULTRY SCIENCE （ 影响因子：3.352； 五年影响因子：3.679 ）

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收录情况： SCI

摘要： Gga-miR-21 is abundantly expressed in chicken pre-adipocytes, but its role is unclear. The present study investigated the role of gga-miR-21 in chicken pre-adipocyte proliferation. Cell proliferation assay and gene expression analysis of proliferating cell nuclear antigen (PCNA) showed that the gga-miR21 mimic inhibited pre-adipocyte proliferation. In contrast, the gga-miR-21 inhibitor enhanced pre-adipocyte proliferation. The subsequent investigation identified Kruppel-like factor 5 (KLF5) mRNA as a target of gga-miR-21. The gga-miR-21 mimic inhibited KLF5 3'UTR reporter activity and decreased endogenous KLF5 expression in primary pre-adipocytes. KLF5 knockdown using RNAi had a similar effect to that of the gga-miR21 mimic on cell proliferation. The promoting effect of the gga-miR-21 inhibitor on pre-adipocyte proliferation was partially attenuated by KLF5 knockdown. Taken together, our results demonstrated that miR-21 inhibits chicken pre-adipocyte proliferation, at least in part, by targeting KLF5.

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