您好，欢迎访问上海市农业科学院 机构知识库！
上海市农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

An ultrasensitive amperometric immunosensor for zearalenones based on oriented antibody immobilization on a glassy carbon electrode modified with MWCNTs and AuPt nanoparticles

文献类型： 外文期刊

作者： Liu, Na 1 ; Nie, Dongxia 2 ; Tan, Yanglan 1 ; Zhao, Zhiyong 2 ; Liao, Yucai 3 ; Wang, Hui 1 ; Sun, Changpo 4 ; Wu, Aibo 1 ;

作者机构： 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Food Safety Res Inst Nutr Sci, SIBS UGENT SJTU Joint Lab Mycotoxin Res, 294 Taiyuan Rd, Shanghai 200031, Peoples R China

2.Shanghai Acad Agr Sci, Inst Agrifood Stand & Testing Technol, 1000 Jinqi Rd, Shanghai 201403, Peoples R China

3.Huazhong Agr Univ, Coll Plant Sci & Technol, 1 Shizishan St, Wuhan 430070, Hubei, Peoples R China

4.Acad State Adm

关键词： Biosensor;Electroanalysis;Mycotoxins;Electrodeposition;Gold nanoparticles;Platinum nanoparticles;Cyclic voltammetry;Differential pulse voltammetry;Food analysis

期刊名称：MICROCHIMICA ACTA （ 影响因子：5.833； 五年影响因子：5.357 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The family of zearalenones (ZENs) represents a major group of mycotoxins with estrogenic activity. They are produced by Fusarium fungi and cause adverse effects on human health and animal production. The authors describe here a label-free amperometric immunosensor for the direct determination of ZENs. A glassy carbon electrode (GCE) was first modified with polyethyleneimine-functionalized multi-walled carbon nanotubes. Next, gold and platinum nanoparticles (AuPt-NPs) were electro-deposited. This process strongly increased the surface area for capturing a large amount of antibodies and enhanced the electrochemical performance. In a final step, monoclonal antibody against zearalenone was orientedly immobilized on the electrode, this followed by surface blocking with BSA. The resulting biosensor was applied to the voltammetry determination of ZENs, best at a working voltage of 0.18 V (vs SCE). Under optimized conditions, the method displays a wide linear range that extends from 0.005 to 50 ng mL(-1), with a limit of detection of 1.5 pg mL(-1) (at an S/N ratio of 3). The assay is highly reproducible and selective, and therefore provides a sensitive and convenient tool for determination of such mycotoxins.

  • 相关文献

[1]Multianalysis of 35 Mycotoxins in Traditional Chinese Medicines by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry Coupled with Accelerated Solvent Extraction. Han, Zheng,Zhu, Junfeng,Chen, Yong,Luan, Lianjun,Wu, Yongjiang,Ren, Yiping,Cai, Zengxuan,Han, Zheng.

[2]Determination of Mycotoxin Biomarkers in Eggs by Liquid Chromatography-Tandem Mass Spectrometry Coupled with Matrix Solid Phase Dispersion. Zhu Run-Yue,Xu Fei,Zhu Run-Yue,Zhao Zhi-Yong,Song Su-Quan,Yang Xian-Li,Nie Dong-Xia,Wu Ai-Bo,Song Su-Quan. 2015

[3]A simple sample pretreatment method for multi-mycotoxin determination in eggs by liquid chromatography tandem mass spectrometry. Zhu, Runyue,Zhao, Zhiyong,Yan, Liping,Song, Suquan,Zhao, Zhiyong,Wang, jianhua,Bai, Bing,Wu, Aibo,Song, Suquan,Wu, Aibo,Zhu, Runyue.

[4]Occurrence and exposure assessment of multiple mycotoxins in dried fruits based on liquid chromatography-tandem mass spectrometry. Han, Z.,Dong, M.,Han, W.,Shen, Y.,Nie, D.,Shi, W.,Zhao, Z.,Han, Z..

[5]A quick, easy, cheap, effective, rugged, and safe sample pretreatment and liquid chromatography with tandem mass spectrometry method for the simultaneous quantification of 33 mycotoxins in Lentinula edodes. Han, Zheng,Feng, Zhihong,Shi, Wen,Zhao, Zhihui,Wu, Aibo,Han, Zheng,Wu, Yongjiang.

[6]Development and application of salting-out assisted liquid/liquid extraction for multi-mycotoxin biomarkers analysis in pig urine with high performance liquid chromatography/tandem mass spectrometry. Song, Suquan,Ediage, Emmanuel Njumbe,De Saeger, Sarah,Song, Suquan,Wu, Aibo.

[7]A reliable liquid chromatography-tandem mass spectrometry method for simultaneous determination of multiple mycotoxins in fresh fish and dried seafoods. Sun, Wenshuo,Han, Zheng,Nie, Dongxia,Jin, Mengtong,Shi, Wen,Zhao, Zhiyong,Wu, Aibo,Sun, Wenshuo,Jin, Mengtong,Zhao, Yong,Aerts, Johan,Han, Zheng,Aerts, Johan,De Saeger, Sarah.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：上海市农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台