Isolation and Characterization of a Phaseolus vulgaris Trypsin Inhibitor with Antiproliferative Activity on Leukemia and Lymphoma Cells
文献类型： 外文期刊
作者： Li, Miao 1 ; Liu, Qin 4 ; Cui, Yajuan 3 ; Li, Dong 3 ; Wang, Hexiang 1 ; Ng, Tzi Bun 5 ;
作者机构： 1.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
2.China Agr Univ, Dept Microbiol, Beijing 100193, Peoples R China
3.Beijing Res Inst Nutr Resources, Engn Res Ctr Syst Nutr, Beijing 100069, Peoples R China
4.Henan Acad Agr Sci, Inst Plant Nutr Agr Resources & Environm Sci, Zhengzhou 450002, Peoples R China
5.Chinese Univ Hong Kong, Sch Biomed Sci, Fac Med, Shatin, Hong Kong, Peoples R China
关键词： leguminous seeds;leukemia L1210;lymphoma MBL2;purification
期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）
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收录情况： SCI
摘要： A 17.5-kDa trypsin inhibitor was purified from Phaseolus vulgaris cv. gold bean with an isolation protocol including ion exchange chromatography on DEAE-cellulose (Diethylaminoethyl-cellulose), affinity chromatography on Affi-gel blue gel, ion exchange chromatography on SP-sepharose (Sulfopropyl-sepharose), and gel filtration by FPLC (Fast protein liquid chromatography) on Superdex 75. It dose-dependently inhibited trypsin with an IC50 value of 0.4 mu M, and this activity was reduced in the presence of dithiothreitol in a dose- and time-dependent manner, signifying the importance of the disulfide linkage to the activity. It inhibited [methyl-H-3] thymidine incorporation by leukemia L1210 cells and lymphoma MBL2 cells with an IC50 value of 2.3 mu M and 2.5 mu M, respectively. The inhibitor had no effect on fungal growth and the activities of various viral enzymes when tested up to 100 mu M.
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