您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Production of biologically active recombinant goose FSH in a single chain form with a CTP linker sequence

文献类型： 外文期刊

作者： Li, Hui 1 ; Zhu, Huanxi 1 ; Qin, Qinming 2 ; Lei, Mingming 1 ; Shi, Zhendan 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Key Lab Anim Breeding & Reprod, Inst Anim Sci, Nanjing 210014, Jiangsu, Peoples R China

2.South China Agr Univ, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China

关键词： Goose;Recombinant FSH;Single chain peptide;Mammalian cell expression;Bioactivity

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： FSH is a glycoprotein hormone secreted by the pituitary gland that is essential for gonadal development and reproductive function. In avian reproduction study, especially in avian reproduction hormone study, it is hindered by the lack of biologically active FSH. In order to overcome this shortcoming, we prepared recombinant goose FSH as a single chain molecule and tested its biological activities in the present study. Coding sequences for mature peptides of goose FSH alpha and beta subunits were amplified from goose pituitary cDNA. A chimeric gene containing alpha and beta subunit sequences linked by the hCG carboxyl terminal peptide coding sequence was constructed. The recombinant gene was inserted into the pcDNA3.1-Fc eukaryotic expression vector to form pcDNA-Fc-gFSH beta-CTP-alpha and then transfected into 293-F cells. A recombinant, single chain goose FSH was expressed and verified by SDS-PAGE and western blot analysis, and was purified using Protein A agarose affinity and gel filtration chromatography. Biological activity analysis results showed that the recombinant, chimeric goose FSH possesses the function of stimulating estradiol secretion and cell proliferation, in cultured chicken granulosa cells. These results indicated that bioactive, recombinant goose FSH has been successfully prepared in vitro. The recombinant goose FSH will have the potential of being used as a research tool for studying avian reproductive activities, and as a standard for developing avian FSH bioassays.

  • 相关文献

[1]Identification and molecular characterization of a novel flavivirus isolated from geese in China. Huang, Xinmei,Han, Kaikai,Zhao, Dongmin,Liu, Yuzhuo,Zhang, Jingfeng,Niu, Huimin,Li, Yin,Zhang, Kangning,Zhu, Jiangning,Wu, Dongming,Gao, Lei.

[2]Development of Double Antibody Sandwich ELISA for Detection of Duck or Goose Flavivirus. Niu Hui-min,Huang Xin-mei,Han Kai-kai,Liu Yu-zhuo,Zhao Dong-min,Zhang Jing-feng,Liu Fei,Li Tong-tong,Zhou Xiao-bo,Li Yin,Niu Hui-min,Liu Fei,Li Tong-tong,Zhou Xiao-bo,Li Xiang-rui. 2013

[3]Genetic variation of Pit-1 gene in Chinese indigenous and Western goose populations. Cheng, Jin-hua,Qiao, Na,Zhao, Wen-ming,Xu, Qi,Zhang, Hai-bo,Duan, Xiu-jun,Chen, Guo-hong,Ji, Wen-lin,Cheng, Jin-hua. 2009

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台