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GPR39 activates proliferation and differentiation of porcine intramuscular preadipocytes through targeting the PI3K/AKT cell signaling pathway

文献类型： 外文期刊

作者： Dong, Xiaoying 1 ; Tang, Shengqiu 1 ; Zhang, Wei 2 ; Gao, Weihua 3 ; Chen, Yanfei 4 ;

作者机构： 1.Shaoguan Univ, Coll Yingdong Agr Sci & Engn, Daxue Rd, Shaoguan 512005, Peoples R China

2.Hubei Acad Agr Sci, Hubei Key Lab Anim Embryo & Mol Breeding, Wuhan, Peoples R China

3.Yangtze Univ, Coll Anim Sci, Jingzhou, Peoples R China

4.Shaoguan Univ, Coll Yingdong Life Sci, Shaoguan, Peoples R China

关键词： Akt;differentiation;GPR39;inhibitor;PI3K;preadipocyte;proliferation

期刊名称：JOURNAL OF RECEPTORS AND SIGNAL TRANSDUCTION （ 影响因子：2.092； 五年影响因子：2.683 ）

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收录情况： SCI

摘要： Background: The orphan G protein-coupled receptor (GPR) 39 was originally identified as the receptor of obestatin. In this study, the effects and mechanisms of GPR39 on cell proliferation and differentiation were investigated in cultured porcine intramuscular preadipocytes. Methods: Morphology of preadipocytes and accumulated lipid droplets within cells were identified by an inverted microscope. After transfected with constructed pCMV-GPR39 plasmid, cell proliferation was measured by using methyl thiazolyl tetrazolium method, mRNA expression of GPR39, CCAAT/enhancer binding protein- (C/EBP), peroxisome proliferator-activated receptor- (PPAR), Caspase-9 and adipocyte determination and differentiation factor-1 (ADD1) was determined by RNA preparation and reverse transcription polymerase chain reaction, protein expression of phosphoinositide-3 kinase (PI3K), 3-phosphoinositide-dependent protein kinase 1, phosphorylated glycogen synthase kinase 3 (pGSK3), total Akt and phosphorylated Akt (pAkt) was analyzed by Western blot. Results: It found that GPR39 mRNA and protein were expressed in porcine intramuscular preadipocytes and its expression was significantly up-regulated after treatment with Zn2+ whose function is found to be mediated by GPR39. Furthermore, over-expression of GPR39 further promoted the optical density value of cells, enhanced mRNA expression of PPAR, C/EBP and ADD1, and inhibited mRNA expression of Caspase-9. Protein expression of pGSK3 and pAkt was also increased by GPR39 stimulation. In addition, GPR39-induced proliferation and differentiation of porcine intramuscular preadipocytes was partially blocked by the Akt inhibitor (PDTC) and the PI3K inhibitor (LY294002). Conclusion: It indicated that GPR39 was a transducer of Zn2+, and enhanced proliferation and differentiation of porcine intramuscular preadipocytes through activation of the PI3K/Akt signaling pathway.

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