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Ultrahigh pressure-assisted enzymatic extraction maximizes the yield of longan pulp polysaccharides and their acetylcholinesterase inhibitory activity in vitro

文献类型： 外文期刊

作者： Bai, Yajuan 1 ; Liu, Lei 2 ; Zhang, Ruifen 2 ; Huang, Fei 2 ; Deng, Yuanyuan 2 ; Zhang, Mingwei 1 ;

作者机构： 1.Huazhong Agr Univ, Sch Food Sci & Technol, Wuhan 410070, Peoples R China

2.Guangdong Acad Agr Sci, Key Lab Funct Foods, Minist Agr, Guangdong Key Lab Agr Prod Proc,Sericultural & Ag, Guangzhou 510610, Guangdong, Peoples R China

3.Guangdong Acad Agr Sci, Key Lab Funct Foods, Minist Agr, Guangdong Key Lab Agr Prod Proc,Sericultural & Ag

关键词： Longan pulp polysaccharides;Ultrahigh pressure-assisted enzymatic treatment;Acetylcholinesterase

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

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收录情况： SCI

摘要： An extraction method employing ultrahigh pressure-assisted enzymatic treatment was developed and optimized by response surface methodology to increase the yield of longan pulp polysaccharides (LP-UE). A maximum polysaccharides yield of 8.55% was obtained under the optimal conditions of 407 MPa ultrahigh pressure" maintained for 6 min with an enzyme to pretreated material ratio of 1:100, an enzymolysis time of 1.7 h and a water to pretreated material ratio of 42 ml/g. Subsequently, the physicochemical properties and acetylcholinesterase (AChE) inhibitory activity of LP-UE were compared to those of longan pulp polysaccharides (LP) extracted by hot water (LP-H), ultrahigh pressure (LP-U) or enzymatic treatment (LP-E). Results demonstrated that the extraction yield, hexuronic acid content and AChE inhibitory activity of LP-UE was the highest among the four LP samples. LP-UE was primarily made up of arabinose, glucose, and galactose and was linked mainly by beta-type glycosidic linkage. The FTIR spectrum of LP-UE was very similar to those of LP-H, LP-U, and LP-E. In summary, ultrahigh pressure-assisted enzymatic treatment is a more efficient technique for extracting LP with considerable improvement of both yield and memory enhancement function. (C) 2016 Published by Elsevier B.V.

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[1]High-level expression and purification of Plutella xylostella acetylcholinesterase in Pichia pastoris and its potential application. Liu Niu,Che Jun,Lai Duo,Wen Jin-jun,Xu Han-hong,Liu Niu,Che Jun,Lai Duo,Wen Jin-jun,Xu Han-hong,Che Jun,Lai Duo. 2017

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