您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Characterization of miRNAs responsive to exogenous ethylene in grapevine berries at whole genome level

文献类型： 外文期刊

作者： Zhao, Fanggui 1 ; Wang, Chen 1 ; Han, Jian 1 ; Zhu, Xudong 1 ; Li, Xiaopeng 1 ; Wang, Xicheng 3 ; Fang, Jinggui 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China

2.Qingdao Agr Univ, Coll Life Sci, Qingdao 266109, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Hort, Nanjing 210014, Jiangsu, Peoples R China

关键词： Grapevine;Berry;MicroRNAs;Exogenous ethylene;High-throughput sequencing;qRT-PCR

期刊名称：FUNCTIONAL & INTEGRATIVE GENOMICS （ 影响因子：3.41； 五年影响因子：3.616 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： MicroRNAs (miRNAs) are critical regulators of various biological and metabolic processes of plants. Numerous miRNAs and their functions have been identified and analyzed in many plants. However, till now, the involvement of miRNAs in the response of grapevine berries to ethylene has not been reported yet. Here, Solexa technology was employed to deeply sequence small RNA libraries constructed from grapevine berries treated with and without ethylene. A total of 124 known and 78 novel miRNAs were identified. Among these miRNAs, 162 miRNAs were clearly responsive to ethylene, with 55 downregulated, 59 upregulated, and 14 unchanged miRNAs detected only in the control. The other 35 miRNAs responsive to ethylene were induced by ethylene and detected only in the ethylene-treated grapevine materials. Expression analysis of 27 conserved and 26 novel miRNAs revealed that 13 conserved and 18 novel ones were regulated by ethylene during the whole development of grapevine berries. High-throughput sequencing and qRT-PCR assays revealed consistent results on the expression results of ethylene-responsive miRNAs. Moreover, 90 target genes for 34 novel miRNAs were predicted, most of which were involved in responses to various stresses, especially like exogenous ethylene treatment. The identified miRNAs may be mainly involved in grapevine berry development and response to various environmental conditions.

  • 相关文献

[1]Computational identification of microRNAs in peach expressed sequence tags and validation of their precise sequences by miR-RACE. Zhang, Yanping,Yu, Huaping,Han, Jian,Song, Changnian,Fang, Jinggui,Yu, Mingliang,Ma, Ruijuan.

[2]A characterization of grapevine of GRAS domain transcription factor gene family. Sun, Xin,Xie, Zhengqiang,Zhang, Cheng,Mu, Qian,Wang, Baoju,Fang, Jinggui,Xie, Zhengqiang,Wu, Weimin.

[3]IDENTIFICATION OF miRNAs IN SWEET POTATO BY SOLEXA SEQUENCING. Bian, X.,Ma, P.,Jia, Z.,Guo, X.,Xie, Y.,E, Z..

[4]Systematic discovery and characterization of stress-related microRNA genes in Oryza sativa. Xie, Kai Bin,Zhou, Xue,Zhang, Tian Hai,Chen, Guo Xiang,Zhou, Xue,Zhang, Bao Long,Chen, Li Ming.

[5]Identification of novel and salt-responsive miRNAs to explore miRNA-mediated regulatory network of salt stress response in radish (Raphanus sativus L.). Sun, Xiaochuan,Xu, Liang,Wang, Yan,Yu, Rugang,Luo, Xiaobo,Gong, Yiqin,Wang, Ronghua,Limera, Cecilia,Liu, Liwang,Sun, Xiaochuan,Xu, Liang,Luo, Xiaobo,Zhu, Xianwen,Zhang, Keyun. 2015

[6]Conserved miRNAs and Their Response to Salt Stress in Wild Eggplant Solanum linnaeanum Roots. Zhuang, Yong,Zhou, Xiao-Hui,Liu, Jun. 2014

[7]Characterization of a microbial community developing during refrigerated storage of vacuum packed Yao meat, a Chinese traditional food. Zhang, Yingtong,Yao, Yongjie,Gao, Leifeng,Xu, Baocai,Yao, Yongjie,Wang, Zhouping,Zhang, Yingtong. 2018

[8]荷花淹水胁迫下实时定量PCR内参基因的验证. 徐君,江君,王欢,姜红卫. 2019

[9]茉莉花实时荧光定量PCR内参基因的筛选与验证. 齐香玉,陈双双,冯景,王华娣,邓衍明. 2020

[10]早熟砂梨‘苏翠1号’与其亲本‘翠冠’‘华酥’成熟果实差异代谢产物及差异基因比较分析. 王宏,杨王莉,蔺经,杨青松,李晓刚,盛宝龙,常有宏. 2022

[11]辣椒细胞质雄性不育相关基因的克隆与表达分析. 刘科伟,王述彬,刁卫平,戈伟. 2017

[12]辣椒胞质雄性不育相关基因的克隆及表达. 王述彬,刁卫平,刘金兵,潘宝贵,戈伟,郭广君. 2014

[13]Computational identification of microRNAs in strawberry expressed sequence tags and validation of their precise sequences by miR-RACE. Han, Jian,Yu, Hua-Ping,Wang, Chen,Liu, Hong,Fang, Jing-Gui,Dong, Qing-Hua,Ge, An-Jing,Zhao, Mi-Zhen.

[14]Evaluation of appropriate reference genes for gene expression studies in pepper by quantitative real-time PCR. Bin, Wang Shu,Wei, Liu Ke,Ping, Diao Wei,Li, Zhi,Wei, Ge,Bing, Liu Jin,Gui, Pan Bao,Jian, Wan Hong,Feng, Chen Jin,Wei, Liu Ke.

[15]Genome-wide analysis of terpene synthases in soybean: Functional characterization of GmTPS3. Huang, Fang,Wang, Xia,Wang, Jiao,Yu, Deyue,Liu, Jianyu,Zhang, Man,Zheng, Rui.

[16]Characterization of the miR165 family and its target gene Pp-ATHB8 in Prunus persica. Zhang, Chunhua,Guo, Lei,Ma, Ruijuan,Yu, Mingliang,Zhang, Yanping,Han, Jian,Li, Xiaoying. 2012

[17]Selection of Reliable Reference Genes for Gene Expression Studies on Rhododendron molle G. Don. Xiao, Zheng,Sun, Xiaobo,Liu, Xiaoqing,Li, Chang,He, Lisi,Chen, Shangping,Su, Jiale. 2016

[18]Characterization of target mRNAs for Prunus persica microRNAs using an integrated strategy of PLM-RACE, PPM-RACE and qRT-PCR. Zhang, Yanping,Han, Jian,Pervaiz, Tariq,Fang, Jinggui,Yu, Mingliang,Ma, Ruijuan. 2014

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台