文献类型： 外文期刊

作者： Hui, Cai ¹ ; Guo, Xiaoxiao ¹ ; Sun, Pengfei ² ; Lin, Hui ³ ; Zhang, Qichun ⁴ ; Liang, Yongchao ⁵ ; Zhao, Yu-Hua ¹ ;

作者机构： 1.Zhejiang Univ, Coll Life Sci, Inst Microbiol, Hangzhou 310058, Zhejiang, Peoples R China

2.Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Jiangsu, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Environm Resource Soil & Fertilizer, Hangzhou 310021, Zhejiang, Peoples R China

4.Zhejiang Univ, Inst Soil & Water Resources & Environm, Hangzhou 310058, Zhejiang, Peoples R China

5.Zhejiang Univ, Minist Educ, Key Lab Environm Remediat & Ecol Hlth, Coll Environm & Resource Sci, Hangzhou 310058, Zhejiang, Peoples R China

关键词： Anammox;N-damo;Fertilizer regime;Upland-wheat soil;Vertical distribution

期刊名称：APPLIED SOIL ECOLOGY （ 影响因子：4.046； 五年影响因子：4.884 ）

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收录情况： SCI

摘要： Anaerobic ammonium oxidation (anammox) and nitrite-dependent anaerobic methane oxidation (n-damo) are two nitrogen-cycle processes that have recently received more attention. However, few studies have been performed on the effects of different fertilizations in upland-cropping soil. Therefore, the objective of this work was to assess the effects of different fertilizations on the distribution and diversity of anammox and n-damo bacteria along the profiles of soil during wheat cropping season of the paddy-upland rotation system. In our study, a five-year fertilization (from 2010 to 2015) was conducted on a paddy-upland rotation field, with four fertilizer treatments: an unfertilized control (CK), chemical fertilizer (CF), pig manure plus 50% chemical fertilizer (PMCF), and rice straw plus 100% chemical fertilizer (SRCF). The quantitative PCR results showed that the total abundance of anammox and n-damo bacteria during the wheat cropping season were lower than those reported previously in paddy soils, and the abundance of anammox bacteria decreased with soil depth, but the abundance of n-damo bacteria remarkably increased. Long-term fertilization reduced the abundance of anammox bacteria in the 040 cm layer and that of n-damo bacteria in the 20-60 cm layer. Furthermore, Illumina high-throughput sequencing of the hzsB and pmoA genes was performed, showing that anammox bacteria were affiliated with "Candidatus Brocadia" and n-damo bacteria were related to "Candidatus Methylomirabilis oxyfera." Diversity analysis demonstrated that the diversity of anammox bacteria increased with soil depth. Among the fertilizer treatments evaluated, pig manure plus 50% chemical fertilizer (PMCF) yielded the highest abundance and lowest diversity of anammox bacteria in the 20-60 cm layer. Moreover, fertilization diversified the community structure of anammox bacteria in deep soil. For n-damo bacteria, a higher diversity was found under chemical fertilization than under other treatments. Redundancy analysis showed that the community structure of anammox was mainly related to of total nitrogen and soil organic carbon and that of n-damo bacteria was mainly associated with NO(3)(-)content. Overall, soil depth and fertilization patterns influenced the distribution and diversity of anammox and n-damo bacteria by changing the soil properties. (C) 2017 Published by Elsevier B.V.