文献类型： 外文期刊

作者： Zhang, Meiwei ¹ ; Ma, Dongyun ¹ ; Ma, Geng ¹ ; Wang, Chenyang ¹ ; Xie, Xudong ¹ ; Kang, Guozhang ¹ ;

作者机构： 1.Henan Agr Univ, State Key Lab Wheat & Maize Crop Sci, Agron Coll, 62 Nongye Rd, Zhengzhou 450002, Henan, Peoples R China

2.Henan Acad Agr Sci, Inst Food Crops, 116 Huayuan Rd, Zhengzhou 450002, Henan, Peoples R China

关键词： Winter wheat;Grain protein fraction;Glutamine synthetase;Gene expression

期刊名称：JOURNAL OF CEREAL SCIENCE （ 影响因子：3.616； 五年影响因子：3.891 ）

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收录情况： SCI

摘要： Glutamine synthetase (GS) plays a central role in plant nitrogen (N) metabolism, which improves crops grain protein content. A pot experiment in field condition was carried out to evaluate GS expression and activity, and grain protein content in high (Wanmail6) and low grain protein (Loumai24) wheat cultivars under two N levels (0.05 and 0.15 g N kg(-1) soil). High nitrogen (HN) resulted in significant increases in GS1 and GS2 expression at 10 days after anthesis (DAA), and higher GS activity during the entire grain filling stage. HN also significantly increased yield, grain protein content and protein fraction (except for glutenin of Luomai24) in two wheat cultivars, which indicated that it increased grain yield and protein content by improving nitrogen metabolism. Wanmail6 showed higher grain protein content, gliadin and glutenin content, and had higher expression level of GS2 both in flag leaves and grains at early grain filling stage. However, Luomai24 had greater yield and higher expression level of GS1. The difference expression of GS2 and GS1 genes indicates they had various contributions to the accumulation of protein and starch in wheat grains, respectively. The results suggest that GS2 would be serving as a potential breeding target for improving wheat quality. (C) 2017 Published by Elsevier Ltd.