文献类型： 外文期刊

作者： Zeng, A. ¹ ; Song, L. ¹ ; Cui, Y. ² ; Yan, J. ¹ ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Vegetable Crops, Jiangsu Key Lab Hort Crop Genet Improvement, Nanjing 210014, Jiangsu, Peoples R China

2.Bur Agr & Anim Husb, Tangshan 064300, Hebei Province, Peoples R China

关键词： Broccoli (Brassica oleracea L. Var. italica);Microspore culture;Embryogenesis;Cell death;Reduced ascorbate;Reduced glutathione

期刊名称：SOUTH AFRICAN JOURNAL OF BOTANY （ 影响因子：2.315； 五年影响因子：2.71 ）

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收录情况： SCI

摘要： Isolated microspore culture (IMC) is an effective alternative technique for the production of doubled haploid (DH) homozygous lines in plant breeding. However, death of most of the cultured cells during culture is a critical obstacle that affects embryo production in the practical application of IMC technology. In this present paper, we evaluated the effects of two antioxidants of both reduced ascorbate (ASC) and reduced glutathione (GSH) onmicrospore viability and the induction of embryogenesis in the IMC of broccoli (Brassica oleracea L. var. italica). The results showed that cell death occurred soon after stress treatment (at 32.5 degrees C for 24 h), and the number of dead cells reached about 80% after 2 d. The addition of 10 mg l(-1) ASC and 20 mg l(-1) GSH significantly decreased microspore mortality, and had a strong effect on the number of embryos produced. In the genotype 'B415', embryo yields increased approximately 1.2-fold and 2.5-fold after the addition of the 10 mg l(-1) ASC and 20 mg l(-1) GSH, respectively. Moreover, the addition of ASC and GSH also influenced positively the rate of plant regeneration, which respectively increased by 4.2% and 9.7% for 'B415'. Taken together, our results show that the induction of embryogenesis in broccoli is strongly affected by the use of ASC or GSH. The effects on embryogenesis induction could confirm the importance of the redox environmental regulation in IMC, specifically at the early phase of IMC. The obtained knowledge may accelerate and guide the application of microspore-derived DH plants as a breeding tool in broccoli. (C) 2017 SAAB. Published by Elsevier B.V. All rights reserved.