文献类型： 外文期刊

作者： Gillis, Annika ¹ ; Guo, Suxia ¹ ; Bolotin, Alexandre ² ; Makart, Lionel ¹ ; Sorokin, Alexei ² ; Mahillon, Jacques ¹ ;

作者机构： 1.UCL, Earth & Life Inst, Lab Food & Environm Microbiol, Croix Sud 2,L7-05-12, B-1348 Louvain La Neuve, Belgium

2.INRA, Micalis UMR1319, F-78350 Jouy En Josas, France

3.AgroParisTech, Micalis UMR1319, F-78350 Jouy En Josas, France

4.Chinese Acad Trop Agr Sci, Haikou Expt Stn, Haikou, Peoples R China

关键词： Israelensis plasmids;pBtic235;Plasmidial prophage;Biopesticide

期刊名称：RESEARCH IN MICROBIOLOGY （ 影响因子：3.992； 五年影响因子：4.061 ）

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收录情况： SCI

摘要： Bacillus thuringiensis has long been recognized to carry numerous extrachromosomal molecules. Of particular interest are the strains belonging to the B. thuringiensis subsp. israelensis lineage, as they can harbor at least seven extrachromosomal molecules. One of these elements seems to be a cryptic molecule that may have been disregarded in strains considered plasmid-less. Therefore, this work focused on this cryptic molecule, named pBtic235. Using different approaches that included transposition-tagging, large plasmid gel electrophoresis and Southern blotting, conjugation and phage-induction experiments, in combination with bioinformatics analyses, it was found that pBtic235 is a hybrid molecule of 235,425 bp whose genome displays potential plasmid- and phage-like modules. The sequence of pBtic235 has been identified in all sequenced genomes of B. thuringiensis subsp. israelensis strains. Here, the pBtic235 sequence was considered identical to that of plasmid pBTHD789-2 from strain HD-789. Despite the fact that the pBtic235 genome possesses 240 putative CDSs, many of them have no homologs in the databases. However, CDSs coding for potential proteins involved in replication, genome packaging and virion structure, cell lysis, regulation of lytic-lysogenic cycles, metabolite transporters, stress and metal resistance, were identified. The candidate plasmidial prophage pBtic235 exemplifies the notable diversity of the extrachromosomal realm found in B. thuringiensis. (C) 2016 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.