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cDNA cloning, characterization and expression analysis of ATP-binding cassette transmembrane transporter in Exopalaemon carinicauda

文献类型： 外文期刊

作者： Zhai, Qian-Qian 1 ; Li, Jian 1 ; Chang, Zhi-Qiang 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Key Lab Sustainable Dev Marine Fisheries, Minist Agr, Yellow Sea Fisheries Res Inst, 106 Nanjing Rd, Qingdao 266071, Peoples R China

2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Agr, Qingdao 266200, Peoples R China

关键词： Exopalaemon carinicauda;ATP-binding cassette transporters;pH;sulfamerazine;quantitative real-time PCR

期刊名称：AQUACULTURE RESEARCH （ 影响因子：2.082； 五年影响因子：2.415 ）

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收录情况： SCI

摘要： ATP-binding cassette (ABC) transporters are integral membrane proteins which carry diverse substrates across biological membranes. In this study, we report the cloning of a full-length, 2468-bp cDNA sequence encoding a gene of the ABC transporter subfamily G (ABCG) from the shrimp Exopalaemon carinicauda (E. carinicauda). The deduced protein, EcABCG, contains 627 amino acid residues, with a theoretical molecular weight of 69.0kDa. Phylogenetic analysis showed that EcABCG shares the highest identity with the homologs of the shrimp species Litopenaeus vannamei and Fenneropenaeus chinensis, and is also closely related to the insect ABC transporter/white proteins. High-level mRNA expression of EcABCG was detected in the tissue of the hepatopancreas and intestine, using a real-time quantitative reverse-transcriptase PCR. The ABC transporter mRNA transcript in the hepatopancreas of E. carinicauda was significantly upregulated after 6h and after 1h of exposure to acidic and alkaline conditions respectively. EcABCG was also induced in the intestine at 1-24 and 3-48h after exposure to acidic and alkaline conditions respectively. Upon exposure to sulfamerazine diets (50, 100 and 200mgkg(-1)) for 3 and 6h, respectively, the mRNA expression of EcABCG was upregulated in the hepatopancreas. EcABCG was also induced in the intestine after exposure to 50, 100 and 200mgkg(-1) sulfamerazine diets for 6, 3 and 12h respectively. These results suggest that the ABCG transporter of E. carinicauda might play a role in coping with pH stress and in exporting xenobiotics.

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