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Antioxidant enzymes and their role in phoxim and carbaryl stress in Caenorhabditis elegans

文献类型： 外文期刊

作者： Han, Yan 1 ; Song, Shaojuan 4 ; Wu, Haihua 1 ; Zhang, Jianzhen 1 ; Ma, Enbo 1 ;

作者机构： 1.Shanxi Univ, Inst Appl Biol, 92 Wucheng Rd, Taiyuan 030006, Shanxi, Peoples R China

2.Shanxi Univ, Sch Life Sci, 92 Wucheng Rd, Taiyuan 030006, Shanxi, Peoples R China

3.Shanxi Key Lab Integrated Pest Management Agr, 92 Wucheng Rd, Taiyuan 030006, Shanxi, Peoples R China

4.Changzhi Med Coll, Basic Med Coll, Changzhi 046000, Shanxi, Peoples R China

关键词： Caenorhabditis elegans;Phoxim;Carbaryl;Antioxidant enzymes;Oxidative stress

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：3.963； 五年影响因子：4.454 ）

ISSN：

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收录情况： SCI

摘要： Pesticide exposure can induce oxidative stress and cause changes to antioxidant enzymes in living organisms. In the present study, the effects of phoxim (an organophosphorus insecticide) and carbaryl (a carbamate insecticide) on antioxidant enzyme activity and gene expression were investigated in the model organism Caenorhabditis elegans. The results show that phoxim exposure can induce superoxide dismutase (SOD) and catalase (CAT) activities and decrease glutathione peroxidase (GPx) activity at lower concentrations. The expression levels of sod-3, sod-5, ctl-1, gpx-6, and gpx-8 were up-regulated after treatment with phoxim. The mRNA expression levels of sod-5, ctl-1 and gpx-6 were increased approximately 70-, 170- and 130-fold, respectively, in the 0.25 mM treatment group compared to the control group. Carbaryl exposure decreased SOD activity and induced CAT and GPx activities. The addition of carbaryl up-regulated the expression of sod-5, ctl-1, ctl-3 and gpx-8. Specifically, ctl-1 expression increased approximately 10-fold, and gpx-8 expression increased < 30-fold in the 0.5 mM treatment group relative to the control group. The transcript level of sod-5 increased > 20-fold, and ctl-3 increased approximately 10-fold in the 1 mM treatment group. The functions of the antioxidant enzymes during oxidative stress caused by the two insecticides were investigated using deletion mutants. The LC50 values phoxim for the of sod-3 (tm760), sod-5 (tm1146), ctl-1 (ok1242), ctl-3 (ok2042) and gpx-8 (tm2108) mutant strains were lower than those observed for the N2 strain. The LC50 values of carbaryl for the ctl-1 (ok1242), ctl-3 (ok2042) and gpx-6 (tm2535) deletion mutant strains decreased in comparison to the N2 strain. The results suggest that these two insecticides caused oxidative stress and changed altered the antioxidant enzyme activities and their gene expressions in C elegans. The sod-3, sod-5, ctl-1, ctl-3, gpx-6, and gpx-8 encoding enzymes may play roles in defending cells from oxidative stress caused by these two insecticides. (C) 2017 Elsevier Inc. All rights reserved.

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