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Optimization of culture medium and temperature for the in vitro germination of oil palm pollen

文献类型： 外文期刊

作者： Lin, Yiyun 1 ; Wang, Yong 2 ; Iqbal, Amjad 3 ; Shi, Peng 2 ; Li, Jing 2 ; Yang, Yaodong 2 ; Lei, Xintao 2 ;

作者机构： 1.Hainan Univ, Coll Agr Sci, Haikou 570228, Peoples R China

2.Chinese Acad Trop Agr Sci, Coconut Res Inst, Wenchang 571339, Peoples R China

3.Abdul Wali Khan Univ Mardan, Dept Agr, Garden Campus, Mardan, Pakistan

关键词： Oil palm;Pollen;Culture medium;Pollen tube length;Germination rate

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN：

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收录情况： SCI

摘要： Pollens from two different types of oil palm, Pisifera and Dura, were used to study the influence of different medium components and temperature on oil palm pollen germination. The results of our study revealed that Murashige and Skoog (MS) and sucrose (0.05% MS+0.01 g/mL sucrose) played a catalytic role in oil palm pollen germination and pollen tube growth. The catalytic effect could be reversed when the contents of the medium were used at higher concentrations. Besides the nutrients, temperature has also played a vital role in vitro germination of oil palm pollen. The recorded optimum temperature and time for the germination was at 31 degrees C for 3 h. A fluctuation in time and temperature has inhibited the germination rate, as well as the growth of the pollen tube to a certain level. Thus, we have concluded from this study that a proper combination of culture medium and temperature could enhance the in vitro germination of oil palm pollen. These results will be helpful in preparing pollens for oil palm pollination. (C) 2017 Elsevier B.V. All rights reserved.

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